Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery
文献类型：期刊论文
| 作者 | Wu, Le-yu; Yu, Xue-li; Feng, Lin-yin
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2015-08 |
| 卷号 | 36期号:8页码:928-938 |
| 关键词 | astrocytes Connexin 43 oxygen-glucose deprivation/recovery middle cerebral artery occlusion CREB Ephrin-A4 calcium |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2015.39 |
| 文献子类 | Article |
| 英文摘要 | Aim: Connexin 43 (Cx43) is a member of connexin family mainly expressed in astrocytes, which forms gap junctions and hemichannels and maintains the normal shape and function of astrocytes. In this study we investigated the role of Cx43 in astrocytes in facilitating neuronal recovery during ischemic stroke. Methods: Primary culture of astrocytes or a mixed culture of astrocytes and cortical neurons was subjected to oxygen glucose deprivation and reperfusion (OGD/R). The expression of Cx43 and Ephrin-A4 in astrocytes was detected using immunocytochemical staining and Western blot assays. Intercellular Ca2+ concentration was determined with Fluo-4 AM fluorescent staining. Middle cerebral artery occlusion (MCAO) model rats were used for in vivo studies. Results: OGD/R treatment of cultured astrocytes caused a decrement of Cx43 expression and translocation of Cx43 from cell membrane to cytoplasm, accompanied by cell retraction. Furthermore, OGD/R increased intracellular Ca2+ concentration, activated CaMKII/CREB pathways and upregulated expression of Ephrin-A4 in the astrocytes. All these changes in OGD/R-treated astrocytes were alleviated by overexpression of Cx43. In the cortical neurons cultured with astrocytes, OGD/R inhibited the neurite growth, whereas overexpression of Cx43 or knockdown of Ephrin-A4 in astrocytes restored the neurite growth. In MCAO model rats, neuronal recovery was found to be correlated with the recuperation of Cx43 and Ephrin-A4 in astrocytes. Conclusion: Cx43 can stabilize astrocytes and facilitate the resistance to the deleterious effects of a stroke-like milieu and promote neuronal recovery. |
| WOS关键词 | SULFATE PROTEOGLYCANS NEUROCAN ; GAP JUNCTIONAL COMMUNICATION ; CULTURED ASTROCYTES ; CORTICAL ASTROCYTES ; NEURITE OUTGROWTH ; CEREBRAL-ISCHEMIA ; CALCIUM INFLUX ; HEMICHANNELS ; EXPRESSION ; CYTOKINES |
| 资助项目 | National Natural Science Foundation of China[81123004] ; Chinese Academy of Sciences[XDA01040304] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2014ZX09102-001-05] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5487142 |
| WOS记录号 | WOS:000358934000004 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276452]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Feng, Lin-yin |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Le-yu,Yu, Xue-li,Feng, Lin-yin. Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery[J]. ACTA PHARMACOLOGICA SINICA,2015,36(8):928-938. |
| APA | Wu, Le-yu,Yu, Xue-li,&Feng, Lin-yin.(2015).Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery.ACTA PHARMACOLOGICA SINICA,36(8),928-938. |
| MLA | Wu, Le-yu,et al."Connexin 43 stabilizes astrocytes in a stroke-like milieu to facilitate neuronal recovery".ACTA PHARMACOLOGICA SINICA 36.8(2015):928-938. |
入库方式： OAI收割
来源：上海药物研究所
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