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Label-Free Proteomics Uncovers Energy Metabolism and Focal Adhesion Regulations Responsive for Endometrium Receptivity

文献类型：期刊论文

作者Chen, Qian1,2; Zhang, Aijun1; Yu, Feng3; Gao, Jing3; Liu, Yue2; Yu, Chengli3; Zhou, Hu3,4; Xu, Chen2
刊名JOURNAL OF PROTEOME RESEARCH
出版日期2015-04
卷号14期号:4页码:1831-1842
关键词endometrium endometrial receptivity label-free proteomic analysis focal adhesion
ISSN号1535-3893
DOI10.1021/acs.jproteome.5b00038
文献子类Article
英文摘要The menstrual cycle of the female uterus leads to periodic changes of the endometrium. These changes are important for developing the endometrial receptivity and for achieving competency of embryo implantation. However, the molecular events underlying the endometrial receptivity process remain poorly understood. Here we applied an LC-MS-based label-free quantitative proteomic approach to compare the endometrial tissues in the midsecretory (receptive) phase with the endometrial tissues in the proliferative phase from age-matched woman (n = 6/group). The proteomes of endometrial tissues were extracted using an SDS-based detergent, digested by the filter-aided sample preparation procedures, and subsequently analyzed by nano-LC-MS/MS (Orbitrap XL) with a 4 h gradient. Reliable protein expression profiles were reproducibly obtained from the endometrial tissues in the receptive and proliferative phases. A total of 2138 protein groups were quantified under highly stringent criteria with a false discovery rate of <1% for peptide and protein groups. Among these proteins, 317 proteins had differences in expression that were statistically significant between the receptive and proliferative phases. Direct protein-protein interaction network analyses of these significantly changed proteins showed that the up-regulation of creatine kinase B-type (CKB) in the receptive phase may be related to endometrium receptivity. The interaction network also showed that proteins related to cell-cell adhesion were down-regulated. Moreover, the results from KEGG pathway analyses are consistent with the protein-protein interaction results. The proteins, including alpha-actinin (ACTN), extracellular matrix proteins, integrin alpha-V, and so on, that are involved in the focal adhesion pathway were down-regulated in the receptive phase compared with the proliferative phase, which may facilitate the implantation of the fertilized ovum. Selected proteins were validated by Western blot analysis and indirect immunofluorescence, including the up-regulation of CKB and down-regulation ACTN in the receptive phase. In summary, our proteomic analysis study shows potential for predicting the endometrial remodeling from the proliferative to the receptivity phase in women, and these results also reveal the key biological mechanisms (such as energy metabolism and focal adhesion) underlying human endometrial receptivity
WOS关键词LEUKEMIA-INHIBITORY-FACTOR ; UTERINE EPITHELIAL-CELLS ; CREATINE-KINASE ACTIVITY ; EMBRYO IMPLANTATION ; PROTEIN EXPRESSION ; GENE-EXPRESSION ; MICROARRAY TECHNOLOGY ; PLASMA-MEMBRANE ; MENSTRUAL-CYCLE ; PERIIMPLANTATION
资助项目National Natural Science Foundation of China[81100469] ; National Natural Science Foundation of China[21375138] ; Chinese Academy of Sciences[00000000] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2013ZX09507001] ; E-institutes of Shanghai Municipal Education Commission[E03008]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000352463300017
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/276585]  
专题分析化学研究室
通讯作者Xu, Chen
作者单位1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Ctr Reprod Med, Shanghai 200025, Peoples R China;
2.Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Reprod Med, Dept Human Anat Histol & Embryol, Shanghai 200025, Peoples R China;
3.Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China;
4.Shanghai Univ Tradit Chinese Med, E Inst Shanghai Municipal Educ Comm, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Qian,Zhang, Aijun,Yu, Feng,et al. Label-Free Proteomics Uncovers Energy Metabolism and Focal Adhesion Regulations Responsive for Endometrium Receptivity[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(4):1831-1842.
APA Chen, Qian.,Zhang, Aijun.,Yu, Feng.,Gao, Jing.,Liu, Yue.,...&Xu, Chen.(2015).Label-Free Proteomics Uncovers Energy Metabolism and Focal Adhesion Regulations Responsive for Endometrium Receptivity.JOURNAL OF PROTEOME RESEARCH,14(4),1831-1842.
MLA Chen, Qian,et al."Label-Free Proteomics Uncovers Energy Metabolism and Focal Adhesion Regulations Responsive for Endometrium Receptivity".JOURNAL OF PROTEOME RESEARCH 14.4(2015):1831-1842.

入库方式： OAI收割

来源：上海药物研究所

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