Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics
文献类型：期刊论文
| 作者 | Hao, Xu1; Liu, Xia2 ; Wang, Weiming1; Ren, Hong1; Xie, Jingyuan1; Shen, Pingyan1; Lin, Donghai2,3; Chen, Nan1
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| 刊名 | PLOS ONE
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| 出版日期 | 2013-11-11 |
| 卷号 | 8期号:11 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0078531 |
| 文献子类 | Article |
| 英文摘要 | Background: Primary focal segmental glomerulosclerosis (FSGS) is pathological entity which is characterized by idiopathic steroid-resistant nephrotic syndrome (SRNS) and progression to end-stage renal disease (ESRD) in the majority of affected individuals. Currently, there is no practical noninvasive technique to predict different pathological types of glomerulopathies. In this study, the role of urinary metabolomics in the diagnosis and pathogenesis of FSGS was investigated. Methods: NMR-based metabolomics was applied for the urinary metabolic profile in the patients with FSGS (n = 25), membranous nephropathy (MN, n = 24), minimal change disease (MCD, n = 14) and IgA nephropathy (IgAN, n = 26), and healthy controls (CON, n = 35). The acquired data were analyzed using principal component analysis (PCA) followed by orthogonal projections to latent structure discriminant analysis (OPLS-DA). Model validity was verified using permutation tests. Results: FSGS patients were clearly distinguished from healthy controls and other three types of glomerulopathies with good sensitivity and specificity based on their global urinary metabolic profiles. In FSGS patients, urinary levels of glucose, dimethylamine and trimethylamine increased compared with healthy controls, while pyruvate, valine, hippurate, isoleucine, phenylacetylglycine, citrate, tyrosine, 3-methylhistidine and b-hydroxyisovalerate decreased. Additionally, FSGS patients had lower urine N-methylnicotinamide levels compared with other glomerulopathies. Conclusions: NMR-based metabonomic approach is amenable for the noninvasive diagnosis and differential diagnosis of FSGS as well as other glomerulopathies, and it could indicate the possible mechanisms of primary FSGS. |
| WOS关键词 | RENAL ORGANIC ANION ; GLYCINE CONJUGATION ; BENZOIC-ACID ; OXIDATIVE STRESS ; UREMIC TOXINS ; RAT-KIDNEY ; METABONOMICS ; 3-METHYLHISTIDINE ; TRANSPORT ; URINE |
| 资助项目 | National Basic Research Program of China 973 Program[2012CB517600] ; National Basic Research Program of China 973 Program[2012CB517604] ; National Natural Science Foundation of China[81070568] ; National Natural Science Foundation of China[81000295] ; National Natural Science Foundation of China[81370015] ; Shanghai Pujiang Program[11PJ1406700] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000327221600031 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277380]  |
| 专题 | 分析化学研究室 药物安全性评价中心 |
| 通讯作者 | Chen, Nan |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Dept Nephrol, Ruijin Hosp, Shanghai 200030, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Bimol NMR Lab, Shanghai 200031, Peoples R China; 3.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Xu,Liu, Xia,Wang, Weiming,et al. Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics[J]. PLOS ONE,2013,8(11). |
| APA | Hao, Xu.,Liu, Xia.,Wang, Weiming.,Ren, Hong.,Xie, Jingyuan.,...&Chen, Nan.(2013).Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics.PLOS ONE,8(11). |
| MLA | Hao, Xu,et al."Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics".PLOS ONE 8.11(2013). |
入库方式： OAI收割
来源：上海药物研究所
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