Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11
文献类型：期刊论文
| 作者 | Gu, Yina1,4; Liu, Qiang3; Chen, Peiyan1; Guo, Chenyun1; Liu, Yan1; Zhao, Yufen1; Zhang, Yonglian3; Lin, Donghai1,2,4 |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
 |
| 出版日期 | 2013-01 |
| 卷号 | 1834期号:1页码:1-7 |
| 关键词 | Lipocalin Lcn11 Dimer Disulfide bond Ligand-binding beta-barrel |
| ISSN号 | 1570-9639 |
| DOI | 10.1016/j.bbapap.2012.08.018 |
| 文献子类 | Article |
| 英文摘要 | Lipocalin 11 (Lcn11), a recently identified member of the lipocalin family, potentially plays crucial physiological roles in male reproduction. In this present work, we cloned, expressed and purified the rat Lcnl11 (rLcn11) protein in Escherichia coli. A C59A/C156A substitution was introduced to ameliorate the misfolding and aggregation problem associated with the wild-type protein. From circular dichroism and non-reducing SDS-PAGE, we characterized the conformational properties of rLcn11 as a typical lipocalin scaffold with the conserved disulfide bridge. The results obtained from size-exclusion chromatography, cross-linking experiment and dynamic light scattering analysis indicate that the recombinant rLcn11 protein forms dimer in neutral solution. By using fluorescent probe 8-anilino-1-naphtahlene sulfonic acid (ANS), we found rLcn11 might contain multiple hydrophobic binding sites for ligand binding. Similarly to the odorant-binding protein, rLcn11 processes a moderate affinity for binding 1-aminoanthracene (AMA), implying that Lcn11 might work as a dimeric chemoreception protein in male reproductive system. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | EQU C 1 ; BETA-LACTOGLOBULIN ; URINARY PROTEINS ; FLUORESCENCE SPECTROSCOPY ; 3-DIMENSIONAL STRUCTURE ; STRUCTURAL-CHANGES ; PHEROMONE-BINDING ; SITES ; DIMER ; IDENTIFICATION |
| 资助项目 | Natural Science Foundation of China[91129713] ; Natural Science Foundation of China[31170717] ; Natural Science Foundation of China[30730026] ; Program of Shanghai Subject Chief Scientist[09XD1405100] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000314620400001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277817]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.Xiamen Univ, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Biomol NMR, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China; 4.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Gu, Yina,Liu, Qiang,Chen, Peiyan,et al. Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2013,1834(1):1-7. |
| APA | Gu, Yina.,Liu, Qiang.,Chen, Peiyan.,Guo, Chenyun.,Liu, Yan.,...&Lin, Donghai.(2013).Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1834(1),1-7. |
| MLA | Gu, Yina,et al."Characterization of the oligomerization and ligand-binding properties of recombinant rat lipocalin 11".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1834.1(2013):1-7. |
入库方式： OAI收割
来源：上海药物研究所
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