H-1 NMR spectroscopy analysis of metabolites in the kidneys provides new insight into pathophysiological mechanisms: applications for treatment with Cordyceps sinensis
文献类型：期刊论文
| 作者 | Zhong, Fang1; Liu, Xia2 ; Zhou, Qiao1; Hao, Xu1; Lu, Ying1; Guo, Shanmai1; Wang, Weiming1; Lin, Donghai2,3; Chen, Nan1
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| 刊名 | NEPHROLOGY DIALYSIS TRANSPLANTATION
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| 出版日期 | 2012-02 |
| 卷号 | 27期号:2页码:556-565 |
| 关键词 | 5 6 nephrectomy Cordyceps sinensis H-1 NMR spectroscopy analysis metabonomics pathophysiology |
| ISSN号 | 0931-0509 |
| DOI | 10.1093/ndt/gfr368 |
| 文献子类 | Article |
| 英文摘要 | The number of patients with chronic kidney disease (CKD) is continuously growing worldwide. Treatment with traditional Chinese medicine might slow the progression of CKD. In this study, we evaluated the renal protective effects of the Chinese herb Cordyceps sinensis in rats with 5/6 nephrectomy. Male Sprague-Dawley mice (weighing 150-200 g) were subjected to 5/6 nephrectomy. The rats were divided into three groups: (i) untreated nephrectomized group (OP group, n = 16), (ii) oral administration of C. sinensis-treated (4 mg/kg/day) nephrectomized group (CS group, n = 16) and (iii) sham-operated group (SO group, n = 16). The rats were sacrificed at 4 and 8 weeks after 5/6 nephrectomy, and the kidneys, serum and urine were collected for H-1 nuclear magnetic resonance spectral analysis. Multivariate statistical techniques and statistical metabolic correlation comparison analysis were performed to identify metabolic changes in aqueous kidney extracts between these groups. Significant differences between these groups were discovered in the metabolic profiles of the biofluids and kidney extracts. Pathways including the citrate cycle, branched-chain amino acid metabolism and the metabolites that regulate permeate pressure were disturbed in the OP group compared to the SO group; in addition, these pathways were reversed by C. sinensis treatment. Biochemistry and electron microscopic images verified that C. sinensis has curative effects on chronic renal failure. These results were confirmed by metabonomics results. Our study demonstrates that C. sinensis has potential curative effects on CKD, and our metabonomics results provided new insight into the mechanism of treatment of this traditional Chinese medicine. |
| WOS关键词 | TRADITIONAL CHINESE MEDICINE ; BIOMARKER IDENTIFICATION ; SUCCINATE-DEHYDROGENASE ; OSMOTIC REGULATION ; METABONOMICS ; MODEL ; DISEASE ; ACCUMULATION ; APOPTOSIS ; CELLS |
| 资助项目 | National Nature Science foundation of China[30270613] ; National Nature Science foundation of China[30771000] ; Foundation of Science and Technology Commission of Shanghai Municipality[08dz1900502] ; Foundation of Science and Technology Commission of Shanghai Municipality[07JC14037] |
| WOS研究方向 | Transplantation ; Urology & Nephrology |
| 语种 | 英语 |
| WOS记录号 | WOS:000300421300016 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278202]  |
| 专题 | 分析化学研究室 药物安全性评价中心 |
| 通讯作者 | Wang, Weiming |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Nephrol, Shanghai 200030, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, NMR Lab, Shanghai 200031, Peoples R China; 3.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Fang,Liu, Xia,Zhou, Qiao,et al. H-1 NMR spectroscopy analysis of metabolites in the kidneys provides new insight into pathophysiological mechanisms: applications for treatment with Cordyceps sinensis[J]. NEPHROLOGY DIALYSIS TRANSPLANTATION,2012,27(2):556-565. |
| APA | Zhong, Fang.,Liu, Xia.,Zhou, Qiao.,Hao, Xu.,Lu, Ying.,...&Chen, Nan.(2012).H-1 NMR spectroscopy analysis of metabolites in the kidneys provides new insight into pathophysiological mechanisms: applications for treatment with Cordyceps sinensis.NEPHROLOGY DIALYSIS TRANSPLANTATION,27(2),556-565. |
| MLA | Zhong, Fang,et al."H-1 NMR spectroscopy analysis of metabolites in the kidneys provides new insight into pathophysiological mechanisms: applications for treatment with Cordyceps sinensis".NEPHROLOGY DIALYSIS TRANSPLANTATION 27.2(2012):556-565. |
入库方式： OAI收割
来源：上海药物研究所
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