H-1-NMR-based metabonomic analysis of metabolic profiling in diabetic nephropathy rats induced by streptozotocin
文献类型：期刊论文
| 作者 | Zhao, Liangcai3,4; Gao, Hongchang2; Lian, Fulin4; Liu, Xia4 ; Zhao, Yongxiang3; Lin, Donghai1,4
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| 刊名 | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
 |
| 出版日期 | 2011-04 |
| 卷号 | 300期号:4页码:F947-F956 |
| 关键词 | nuclear magnetic resonance metabonomics pathogenic process energy metabolism |
| ISSN号 | 1931-857X |
| DOI | 10.1152/ajprenal.00551.2010 |
| 文献子类 | Article |
| 英文摘要 | Zhao L, Gao H, Lian F, Liu X, Zhao Y, Lin D. H-1-NMR-based metabonomic analysis of metabolic profiling in diabetic nephropathy rats induced by streptozotocin. Am J Physiol Renal Physiol 300: F947-F956, 2011. First published January 12, 2011; doi:10.1152/ajprenal.00551.2010.-Elucidation of the metabolic profiling in diabetic nephropathy (DN) rats is of great assistance for understanding the pathogenesis of DN. In this study, H-1-nuclear magnetic resonance (NMR)-based metabonomics combined with HPLC measurements was used to quantitatively analyze the metabolic changes in urine and kidney extracts from diabetic 2-wk and 8-wk rats induced by streptozotocin (STZ). Pattern recognition analysis of either urine or kidney extracts indicated that the two diabetic groups were separated obviously from the control group, suggesting that the metabolic profiles of the diabetic groups were markedly different from the control. The diabetic 8-wk rats showed lower levels of creatine, dimethylamine, and higher levels of ascorbate, succinate, lactate, citrate, allantoin, 2-ketoglutarate, and 3-hydrobutyrate (3-HB) in the urine samples. Moreover, the diabetic 8-wk rats displayed lower levels of succinate, creatine, myo-inositol, alanine, lactate, and ATP, and higher levels of 3-HB and glucose in the kidney extracts. The observed metabolic changes imply the enhanced pathways of either lipid or ketone body synthesis and decreased pathways of either tricarboxylic acid cycle or glycolysis in DN rats compared with the control. Our results suggest that the energy metabolic changes are associated with the pathogenic process of DN. |
| WOS关键词 | DISEASE ; MODELS ; INJURY ; CELLS ; DEHYDROASCORBATE ; HYPERGLYCEMIA ; HIPPOCAMPUS ; CLEARANCE ; MELLITUS ; REVEALS |
| 资助项目 | National Natural Science Foundation of China[20705037] ; National Natural Science Foundation of China[30730026] ; Chinese Academy of Sciences[KSCX2-YW-R-118] ; Chinese Academy of Sciences[KSCX2-YW-R-104] ; Program of Shanghai[09XD1405100] |
| WOS研究方向 | Physiology ; Urology & Nephrology |
| 语种 | 英语 |
| WOS记录号 | WOS:000289095300017 |
| 出版者 | AMER PHYSIOLOGICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278571]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.Xiamen Univ, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China; 2.Wenzhou Med Coll, Sch Pharm, Wenzhou, Peoples R China; 3.Guangxi Med Univ, Affiliated Hosp 1, Guangxi, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhao, Liangcai,Gao, Hongchang,Lian, Fulin,et al. H-1-NMR-based metabonomic analysis of metabolic profiling in diabetic nephropathy rats induced by streptozotocin[J]. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY,2011,300(4):F947-F956. |
| APA | Zhao, Liangcai,Gao, Hongchang,Lian, Fulin,Liu, Xia,Zhao, Yongxiang,&Lin, Donghai.(2011).H-1-NMR-based metabonomic analysis of metabolic profiling in diabetic nephropathy rats induced by streptozotocin.AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY,300(4),F947-F956. |
| MLA | Zhao, Liangcai,et al."H-1-NMR-based metabonomic analysis of metabolic profiling in diabetic nephropathy rats induced by streptozotocin".AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY 300.4(2011):F947-F956. |
入库方式： OAI收割
来源：上海药物研究所
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