1H NMR-Based Metabonomic Analysis of Metabolic Changes of Serum and Liver in Zucker Obese Rats
文献类型：期刊论文
| 作者 | Zhao, Liangcai2; Zhang, Xiaodong2; Wang, Heyao2 ; Lin, Donghai1,2
|
| 刊名 | ANALYTICAL LETTERS
 |
| 出版日期 | 2011 |
| 卷号 | 44期号:9页码:1579-1590 |
| 关键词 | Insulin resistance Metabonomics Nuclear magnetic resonance (NMR) Type 2 diabetes mellitus (T2DM) Zucker obese rat |
| ISSN号 | 0003-2719 |
| DOI | 10.1080/00032719.2010.520390 |
| 文献子类 | Article |
| 英文摘要 | Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance, which is the inability of tissues to respond properly to normal circulating concentrations of insulin in the body. As an extensively used model of insulin resistance and obesity, the Zucker obese rat exhibits an abnormal regulation of glucose, lipids, and other substances metabolized. In this present work, we applied a 1H nuclear magnetic resonance (NMR)-based metabonomic approach to establish metabolic profiles of both sera and liver extracts from Zucker obese rats and addressed the pathological features of T2DM. Principal component analysis (PCA) demonstrated that Zucker obese rats were separated from the control rats in the levels of sera and aqueous soluble liver extracts, indicating that the metabolic characteristics of the two groups are markedly different. Compared with Zucker lean rats, Zucker obese rats displayed higher sera levels of lactate, N-acetylglycoprotein (NAC), trimethylamine, 3-hydrobutyrate (3-HB), acetoacetate, and lipid. Moreover, Zucker obese rats also showed increased levels of alanine, lactate, creatinine, and decreased levels of betaine, glutamate, acetoacetate, 3-HB, and creatine in aqueous soluble liver extracts. Our results reveal significant alterations of both lipid and energy metabolisms, which reflect the pathological features of T2DM. This work may be of benefit to the understanding of the detailed molecular mechanism of T2DM. |
| WOS关键词 | DEPENDENT DIABETES-MELLITUS ; SKELETAL-MUSCLE ; BLOOD-PLASMA ; SPECTROSCOPY ; FA/FA ; HEPATOTOXICITY ; EXPRESSION ; RESPONSES ; TOXICITY ; CAPACITY |
| 资助项目 | Chinese Academy of Sciences (CAS)[KSCX2-YW-R-118] ; National Science & Technology Major Project[2009ZX09301] ; National Science & Technology Major Project[2009ZX09501-001] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000291805200003 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278630]  |
| 专题 | 药理学第三研究室 分析化学研究室 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhao, Liangcai,Zhang, Xiaodong,Wang, Heyao,et al. 1H NMR-Based Metabonomic Analysis of Metabolic Changes of Serum and Liver in Zucker Obese Rats[J]. ANALYTICAL LETTERS,2011,44(9):1579-1590. |
| APA | Zhao, Liangcai,Zhang, Xiaodong,Wang, Heyao,&Lin, Donghai.(2011).1H NMR-Based Metabonomic Analysis of Metabolic Changes of Serum and Liver in Zucker Obese Rats.ANALYTICAL LETTERS,44(9),1579-1590. |
| MLA | Zhao, Liangcai,et al."1H NMR-Based Metabonomic Analysis of Metabolic Changes of Serum and Liver in Zucker Obese Rats".ANALYTICAL LETTERS 44.9(2011):1579-1590. |
入库方式： OAI收割
来源：上海药物研究所
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