Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore
文献类型：期刊论文
| 作者 | Ye, Sheng4; Li, Yang3 ; Jiang, Youxing1,2
|
| 刊名 | NATURE STRUCTURAL & MOLECULAR BIOLOGY
 |
| 出版日期 | 2010-08 |
| 卷号 | 17期号:8页码:1019-1865 |
| ISSN号 | 1545-9993 |
| DOI | 10.1038/nsmb.1865 |
| 文献子类 | Article |
| 英文摘要 | K+ channels are highly selective for K+ over Na+. Here we present several crystal structures of the MthK K+ channel pore at up to 1.45 angstrom resolution. The MthK selectivity filter maintains a conductive conformation even in the absence of K+, allowing the channel to conduct Na+. The high-resolution structures, along with single-channel recordings, allow for an accurate analysis of how K+ competes with Na+ in a conductive selectivity filter. At high K+ concentrations, two K+ ions equivalently occupy the four sites in the selectivity filter, whereas at low K+/high Na+ concentrations, a single K+ ion remains bound in the selectivity filter, preferably at site 1 or site 3. This single K+ binding at low concentration effectively blocks the permeation of Na+, providing a structural basis for the anomalous mole-fraction effect, a key property of multi-ion pores. |
| WOS关键词 | POTASSIUM-CHANNEL ; CRYSTAL-STRUCTURE ; CALCIUM CHANNELS ; ELECTRON-DENSITY ; ION SELECTIVITY ; NAK CHANNEL ; CONDUCTION ; PERMEATION ; BINDING ; FILTER |
| 资助项目 | US Department of Energy, Office of Energy Research[00000000] ; Howard Hughes Medical Institute[00000000] ; US National Institutes of Health, National Institute of General Medical Science[RO1 GM071621] ; David and Lucile Packard Foundation[00000000] ; Welch Foundation[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280670300020 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278822]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Jiang, Youxing |
| 作者单位 | 1.Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA; 2.Univ Texas SW Med Ctr Dallas, Dept Physiol, Dallas, TX 75390 USA; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China; 4.Zhejiang Univ, Inst Life Sci, Hangzhou, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Sheng,Li, Yang,Jiang, Youxing. Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2010,17(8):1019-1865. |
| APA | Ye, Sheng,Li, Yang,&Jiang, Youxing.(2010).Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore.NATURE STRUCTURAL & MOLECULAR BIOLOGY,17(8),1019-1865. |
| MLA | Ye, Sheng,et al."Novel insights into K+ selectivity from high-resolution structures of an open K+ channel pore".NATURE STRUCTURAL & MOLECULAR BIOLOGY 17.8(2010):1019-1865. |
入库方式： OAI收割
来源：上海药物研究所
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