Fast determination of five components of coumarin, alkaloids and bibenzyls in Dendrobium spp. using pressurized liquid extraction and ultra-performance liquid chromatography
文献类型：期刊论文
| 作者 | Xu, Jun1; Zhao, Wei-min2 ; Qian, Zheng-ming1; Guan, Jia1; Li, Shao-ping1
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2010-06 |
| 卷号 | 33期号:11页码:1580-1586 |
| 关键词 | Dendrobium Fingerprint Pressurized liquid extraction Ultraperformance liquid chromatography |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201000034 |
| 文献子类 | Article |
| 英文摘要 | A fast method for simultaneous determination of five compounds (one coumarin, two alkaloids and two bibenzyls) in Dendrobium spp. using pressurized liquid extraction and ultra-performance liquid chromatography coupled with photo diode array detection was developed. The separation was performed on an Acquity UPLC BEH C-18 column (50 mm x 2.1 mm id, 1.7 mu m) with gradient elution of phosphate buffer (pH = 7.1, 20 mM) and acetonitrile within 6 min. The method was validated for linearity, LOD and LOQ, precision and accuracy. All calibration curves showed good linearity (R-2 >= 0.9999) within test ranges. The overall intra- and inter-day variations (RSDs) of five analytes were less than 2.5%, and the detection recoveries were between 95.6 and 102.3%. The developed method was successfully applied to quantitative analysis of five investigated compounds in different species of Dendrobium. The results showed that there were great variations of their contents, though all these materials were officially used as Chinese herb, Shihu. In addition, the chromatographic fingerprints of Dendrobium spp. were also investigated. |
| WOS关键词 | DIODE-ARRAY DETECTION ; MASS-SPECTROMETRY ; RAPID METHOD ; FINGERPRINT ; PHENOLS ; NOBILE |
| 资助项目 | Science and Technology Development Fund of Macao[028/2006/A2] ; University of Macau[UL015/09-Y1] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000278829700007 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278890]  |
| 专题 | 天然药物化学研究室 |
| 通讯作者 | Li, Shao-ping |
| 作者单位 | 1.Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Phytochem, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jun,Zhao, Wei-min,Qian, Zheng-ming,et al. Fast determination of five components of coumarin, alkaloids and bibenzyls in Dendrobium spp. using pressurized liquid extraction and ultra-performance liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(11):1580-1586. |
| APA | Xu, Jun,Zhao, Wei-min,Qian, Zheng-ming,Guan, Jia,&Li, Shao-ping.(2010).Fast determination of five components of coumarin, alkaloids and bibenzyls in Dendrobium spp. using pressurized liquid extraction and ultra-performance liquid chromatography.JOURNAL OF SEPARATION SCIENCE,33(11),1580-1586. |
| MLA | Xu, Jun,et al."Fast determination of five components of coumarin, alkaloids and bibenzyls in Dendrobium spp. using pressurized liquid extraction and ultra-performance liquid chromatography".JOURNAL OF SEPARATION SCIENCE 33.11(2010):1580-1586. |
入库方式： OAI收割
来源：上海药物研究所
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