The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity
文献类型：期刊论文
| 作者 | Yao, Wenming1,2; Peng, Yu2; Lin, Donghai2,3 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2010-05-28 |
| 卷号 | 396期号:2页码:323-328 |
| 关键词 | ARID JARID1B/PLU-1 EMSA Protein-DNA interaction NMR Intrinsic fluorescence |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2010.04.091 |
| 文献子类 | Article |
| 英文摘要 | JARID1B, a member of the JmjC demethylase family, has a crucial role in H3K4me3 demethylation. The ARID domain is a potential DNA-binding domain of JARID1B. Previous studies indicate that a GC-rich DNA motif is the specific target of the ARID domain. However, the details of the interaction between the ARID domain and duplex DNA require further study. Here, we utilized NMR spectroscopy to assign the backbone amino acids and mapped the DNA-binding sites of the human JARID1B ARID domain. Perturbations to H-1-N-15 correlation spectra revealed that the flexible loop L1 of ARID was the main DNA-binding interface. EMSA and intrinsic fluorescence experiments demonstrated that mutations on loop L1 strongly reduced the DNA-binding activity of JARID1B ARID. Furthermore, transfection of mutant forms resulted in a distinct loss of intrinsic H3K4 demethylase activity, implying that the flexible loop L1 made a major contribution to sustaining the DNA-binding ability of JARID1B ARID domain. (C) 2010 Elsevier Inc. All rights reserved. |
| WOS关键词 | BREAST-CANCER ; DEAD RINGER ; HISTONE DEMETHYLATION ; CONTAINING PROTEINS ; SEQUENCE ; FAMILY ; PLU-1 ; REPRESSION ; MOTIF |
| 资助项目 | Natural Science Foundation of China[30730026] ; Chinese Academy of Sciences (CAS)[KSCX2-YW-R-104] ; Shanghai Institute of Materia Medica, CAS[07G603N058] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000278658000025 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278896]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.E China Normal Univ, Key Lab Opt & Magnet Resonance Spect, Shanghai 200062, Peoples R China; 2.Chinese Acad Sci, NMR Lab, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Xiamen Univ, Key Lab Chem Biol Fujian Prov, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Wenming,Peng, Yu,Lin, Donghai. The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,396(2):323-328. |
| APA | Yao, Wenming,Peng, Yu,&Lin, Donghai.(2010).The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,396(2),323-328. |
| MLA | Yao, Wenming,et al."The flexible loop L1 of the H3K4 demethylase JARID1B ARID domain has a crucial role in DNA-binding activity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 396.2(2010):323-328. |
入库方式： OAI收割
来源：上海药物研究所
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