Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6
文献类型：期刊论文
| 作者 | Guo, Chenyun2; Lian, Yandong2; Liu, Qiang3; Liu, Jiafu2; Zhang, Yonglian3; Lin, Donghai1,2 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2010-01 |
| 卷号 | 69期号:1页码:64-67 |
| 关键词 | Lipocalin6 Soluble expression Stability GB1 Circular dichroism Fluorescence |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2009.07.001 |
| 文献子类 | Article |
| 英文摘要 | Mouse lipocalin6 (mLcn6) was recently identified to be specifically expressed in the epididymis and speculated to may play a role in sperm maturation. However, further studies were hindered due to the bottleneck to obtain enough recombinant mLcn6 proteins. In this article, GB1 tag was successfully applied to improve the soluble expression of mLcn6. Thermal unfolding experiments demonstrate that GB1 can enhance the structural stability of mLcn6. Fluorescence spectroscopy experiments show that mLcn6 prepared according to our procedure has high affinities to both retinoic acid (K-d = 810 nM) and retinol (K-d = 210 nM). In conclusion, soluble, stable and active mLcn6 was recombinantly prepared with the help of the GB1 tag, which will facilitate the structural and functional studies of mLcn6. (C) 2009 Elsevier Inc. All rights reserved. |
| WOS关键词 | RETINOL-BINDING-PROTEIN ; PROSTAGLANDIN D SYNTHASE ; MARKER ; PURIFICATION ; REGION ; CELLS ; LIVER |
| 资助项目 | Shanghai Science and Technology Foundation[07ZR 14135] ; Chinese Natural Science Foundation[30730026] ; Chinese Academy of Sciences[KSCX1-YW-R-54] ; Chinese Academy of Sciences[KSCX2-YW-R-104] ; Chinese 973 programs[2007CB914304] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000272103200010 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279057]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.Xiamen Univ, Dept Biol Chem, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Guo, Chenyun,Lian, Yandong,Liu, Qiang,et al. Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6[J]. PROTEIN EXPRESSION AND PURIFICATION,2010,69(1):64-67. |
| APA | Guo, Chenyun,Lian, Yandong,Liu, Qiang,Liu, Jiafu,Zhang, Yonglian,&Lin, Donghai.(2010).Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6.PROTEIN EXPRESSION AND PURIFICATION,69(1),64-67. |
| MLA | Guo, Chenyun,et al."Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6".PROTEIN EXPRESSION AND PURIFICATION 69.1(2010):64-67. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。