The First Gene-encoded Amphibian Neurotoxin
文献类型：期刊论文
| 作者 | You, Dewen3,5; Hong, Jing4,5; Rong, Mingqiang1; Yu, Haining2; Liang, Songping1; Ma, Yufang3,5; Yang, Hailong3,5; Wu, Jing3,5; Lin, Donghai4; Lai, Ren3 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2009-08-14 |
| 卷号 | 284期号:33页码:22079-22086 |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.M109.013276 |
| 文献子类 | Article |
| 英文摘要 | Many gene-encoded neurotoxins with various functions have been discovered in fish, reptiles, and mammals. A novel 60-residue neurotoxin peptide (anntoxin) that inhibited tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channel (VGSC) was purified and characterized from the skin secretions of the tree frog Hyla annectans (Jerdon). This is the first gene-encoded neurotoxin found in amphibians. The IC50 of anntoxin for the TTX-S channel was about 3.4 mu M. Anntoxin shares sequence homology with Kunitz-type toxins but contains only two of three highly conserved cysteine bridges, which are typically found in these small, basic neurotoxin modules, i.e. snake dendrotoxins. Anntoxin showed an inhibitory ability against trypsin with an inhibitory constant (K-i) of 0.025 mu M. Anntoxin was distributed in skin, brain, stomach, and liver with a concentration of 25, 7, 3, and 2 mu g/g wet tissue, respectively. H. annectans lives on trees or other plants for its entire life cycle, and its skin contains the largest amount of anntoxin, which possibly helps defend against various aggressors or predators. A low dose of anntoxin was found to induce lethal toxicity for several potential predators, including the insect, snake, bird, and mouse. The tissue distribution and functional properties of the current toxin may provide insights into the ecological adaptation of tree-living amphibians. |
| WOS关键词 | SPIDER SELENOCOSMIA-HUWENA ; TOAD BOMBINA-MAXIMA ; ANTIMICROBIAL PEPTIDES ; SODIUM-CHANNEL ; SKIN SECRETIONS ; NA+-CHANNELS ; TETRODOTOXIN ; MUSCLE ; VENOM ; PURIFICATION |
| 资助项目 | Chinese National Natural Science Foundation[30830021] ; Chinese Academy of Sciences[KSCXZ-YW-R-088] ; Chinese Academy of Sciences[KSCX2-YW-G-024] ; Ministry of Science[2007AA02Z192] ; Ministry of Science[2007AA100602] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000268783700032 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279157]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Lin, Donghai |
| 作者单位 | 1.Hunan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Prot Chem & Dev Biol, Changsha 410081, Peoples R China; 2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Hebei, Peoples R China; 3.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Biotoxin Units, Kunming 650223, Yunnan, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 5.Chinese Acad Sci, Grad Sch, Beijing 100009, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Dewen,Hong, Jing,Rong, Mingqiang,et al. The First Gene-encoded Amphibian Neurotoxin[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2009,284(33):22079-22086. |
| APA | You, Dewen.,Hong, Jing.,Rong, Mingqiang.,Yu, Haining.,Liang, Songping.,...&Lai, Ren.(2009).The First Gene-encoded Amphibian Neurotoxin.JOURNAL OF BIOLOGICAL CHEMISTRY,284(33),22079-22086. |
| MLA | You, Dewen,et al."The First Gene-encoded Amphibian Neurotoxin".JOURNAL OF BIOLOGICAL CHEMISTRY 284.33(2009):22079-22086. |
入库方式： OAI收割
来源：上海药物研究所
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