Periplocoside A prevents experimental autoimmune encephalomyelitis by suppressing IL-17 production and inhibits differentiation of Th17 cells
文献类型：期刊论文
| 作者 | Zhang, Jing1; Ni, Jia1; Chen, Zhen-hua3; Li, Xin1; Zhang, Ru-jun3; Tang, Wei1 ; Zhao, Wei-min3; Yang, Yi-fu2; Zuo, Jian-ping1,2
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2009-08 |
| 卷号 | 30期号:8页码:1144-1152 |
| 关键词 | P sepium Bge Periplocoside A experimental autoimmune encephalomyelitis Th17 cells |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2009.101 |
| 文献子类 | Article |
| 英文摘要 | Aim: The aim of this study was to determine the therapeutic effect of Periplocoside A (PSA), a natural product isolated from the traditional Chinese herbal medicine Periploca sepium Bge, in MOG(35-55) (myelin oligodendrocyte glycoprotein 35-55)-induced experimental autoimmune encephalomyelitis (EAE). Methods: Female C57BL/6 mice immunized with MOG(35-55) were treated with (50 mg/kg or 25 mg/kg) or without PSA following immunization and continuously throughout the study. The degree of CNS inflammation was evaluated by H&E staining. Anti-MOG-specific recall responses were analyzed by [H-3]-Thymidine incorporation, ELISA, and RT-PCR. The proportion of IL-17-producing T cells was measured by flow cytometry. Results: Oral administration of PSA significantly reduced the incidence and severity of EAE, which closely paralleled the inhibition of MOG(35-55)-specific IL-17 production. Importantly, PSA inhibited the transcription of IL-17 mRNA and ROR gamma t. Further studies examining intracellular staining and adoptive transfer EAE validated the direct suppressive effect of PSA on Th17 cells. In vitro studies also showed that PSA significantly inhibited the differentiation of Th17 cells from murine purified CD4(+) T cells in a dose-dependent manner. Conclusion: PSA ameliorated EAE by suppressing IL-17 production and inhibited the differentiation of Th17 cells in vitro. Our results provide new insight into the potential mechanisms underlying the immunosuppressive and anti-inflammatory effects of PSA. |
| WOS关键词 | COLLAGEN-INDUCED ARTHRITIS ; CENTRAL-NERVOUS-SYSTEM ; REGULATORY T-CELLS ; IFN-GAMMA ; IL-6-DEFICIENT MICE ; HELPER-CELLS ; IL-23 DRIVES ; SEPIUM BGE ; IN-VIVO ; ACTIVATION |
| 资助项目 | National Natural Science Foundation of China[20672124] ; Science and Technology Commission of Shanghai Municipality[06DZ19721] ; Science and Technology Commission of Shanghai Municipality[08XD14053] ; Science and Technology Commission of Shanghai Municipality[08DZ1980200] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3653582 |
| WOS记录号 | WOS:000269016100010 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279163]  |
| 专题 | 天然药物化学研究室 药理学第一研究室 |
| 通讯作者 | Zhao, Wei-min |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab New Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Prod Chem, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Jing,Ni, Jia,Chen, Zhen-hua,et al. Periplocoside A prevents experimental autoimmune encephalomyelitis by suppressing IL-17 production and inhibits differentiation of Th17 cells[J]. ACTA PHARMACOLOGICA SINICA,2009,30(8):1144-1152. |
| APA | Zhang, Jing.,Ni, Jia.,Chen, Zhen-hua.,Li, Xin.,Zhang, Ru-jun.,...&Zuo, Jian-ping.(2009).Periplocoside A prevents experimental autoimmune encephalomyelitis by suppressing IL-17 production and inhibits differentiation of Th17 cells.ACTA PHARMACOLOGICA SINICA,30(8),1144-1152. |
| MLA | Zhang, Jing,et al."Periplocoside A prevents experimental autoimmune encephalomyelitis by suppressing IL-17 production and inhibits differentiation of Th17 cells".ACTA PHARMACOLOGICA SINICA 30.8(2009):1144-1152. |
入库方式： OAI收割
来源：上海药物研究所
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