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Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Ma, Yaqian2,4; Liu, Menghan1; Tan, Ting3; Yan, Aiping3; Guo, Lan3; Jiang, Kun4; Tan, Changheng4 ; Wan, Yiqun2,3
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| 刊名 | PHYTOCHEMICAL ANALYSIS
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| 出版日期 | 2018-11 |
| 卷号 | 29期号:6页码:639-648 |
| ISSN号 | 0958-0344 |
| 关键词 | Box-Behnken design Camellia oleifera flowers deep eutectic solvents flavonoids response surface methodology |
| DOI | 10.1002/pca.2777 |
| 文献子类 | Article |
| 英文摘要 | Introduction: Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. Objective: To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection. Methodology: Preparing an environmentally-friendly and effective solvent - deep eutectic solvents (DESs) - for compound extraction. Then investigating the influential factors of ultrasound-assisted extraction with DESs by the Box-Behnken design combined with response surface methodology. Results: DES-5 synthesised with choline chloride and lactic acid (1:2) acquired excellent extractability for four flavonoids (quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside and their aglycones) with different polarity. The proposed method, which could simultaneously determine four flavonoids with HPLC-UV detection for the first time, displays satisfactory recovery yields and high precision with inter-day relative standard deviation lower than 5.80%. Conclusion: DESs could be promising solvents for efficiently and selectively extracting bioactive compounds from plant materials, and the analytical method for flavonoids of C. oleifera flowers could provide reference value for its application and be used in other plant resources. |
| WOS关键词 | PHENOLIC-COMPOUNDS ; EFFICIENT EXTRACTION ; BIOACTIVE FLAVONOIDS ; CHOLINE CHLORIDE ; GREEN EXTRACTION ; OPTIMIZATION ; QUERCETIN ; MICROEXTRACTION ; ULTRASOUND ; GLYCOSIDES |
| 资助项目 | Natural Science Foundation of P. R. China[31660483] ; Research Programme of the State Key Laboratory of Food Science and Technology in Nanchang University[SKLF-KF-201617] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000446458200010 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279524]  |
| 专题 | 天然药物化学研究室 |
| 通讯作者 | Tan, Changheng; Wan, Yiqun |
| 作者单位 | 1.Nanchang Univ, Clin Med Coll 2, Nanchang, Jiangxi, Peoples R China; 2.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China; 3.Nanchang Univ, Ctr Anal & Testing, 235 East Nanjing Rd, Nanchang 330029, Jiangxi, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Med Chem, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ma, Yaqian,Liu, Menghan,Tan, Ting,et al. Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography[J]. PHYTOCHEMICAL ANALYSIS,2018,29(6):639-648. |
| APA | Ma, Yaqian.,Liu, Menghan.,Tan, Ting.,Yan, Aiping.,Guo, Lan.,...&Wan, Yiqun.(2018).Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography.PHYTOCHEMICAL ANALYSIS,29(6),639-648. |
| MLA | Ma, Yaqian,et al."Deep eutectic solvents used as extraction solvent for the determination of flavonoids from Camellia oleifera flowers by high-performance liquid chromatography".PHYTOCHEMICAL ANALYSIS 29.6(2018):639-648. |
入库方式： OAI收割
来源：上海药物研究所
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