SILAC-based quantitative proteomic analysis of the livers of spontaneous obese and diabetic rhesus monkeys
文献类型：期刊论文
| 作者 | Wang, Junlong1,2,3; Xu, Shimeng4; Gao, Jing5,6; Zhang, Linqiang1,3; Zhang, Zhiguo1,3; Yang, Wenhui7; Li, Yunhai1,3; Liao, Shasha1,3; Zhou, Hu5,6 ; Liu, Pingsheng4
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| 刊名 | AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
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| 出版日期 | 2018-08 |
| 卷号 | 315期号:2页码:E294-E306 |
| 关键词 | liver rhesus monkey stable isotope labeling with amino acids in cell culture-based quantitative proteomics spontaneous Type 2 diabetes |
| ISSN号 | 0193-1849 |
| DOI | 10.1152/ajpendo.00016.2018 |
| 文献子类 | Article |
| 英文摘要 | Type 2 diabetes mellitus (T2DM) is a severe metabolic disorder that affects more than 10% of the population worldwide. Obesity is a major cause of insulin resistance and contributes to the development of T2DM. Liver is an essential metabolic organ that plays crucial roles in the pathogenesis of obesity and diabetes. However, the underlying mechanisms of liver in the transition of obesity to diabetes are not fully understood. The nonhuman primate rhesus monkey is an appropriate animal for research of human diseases. Here, we first screened and selected three individuals of spontaneously diabetic rhesus monkeys. Interestingly, the diabetic monkeys were obese with a high body mass index at the beginning, but gradually lost their body weight during one year of observation. Furthermore, we performed stable isotope labeling with amino acids in cell culture-based quantitative proteomics to identify proteins and signaling pathways with altered expression in the liver of obese and diabetic monkeys. In total, 3,509 proteins were identified and quantified, of which 185 proteins displayed an altered expression level. Gene ontology analysis revealed that the expression of proteins involved in fatty acids beta-oxidation and galactose metabolism was increased in obese monkeys; while proteins involved in oxidative phosphorylation and branched chain amino acid (BCAA) degradation were upregulated in diabetic monkeys. In addition, we observed mild apoptosis in the liver of diabetic monkeys, suggesting liver injury at the late onset of diabetes. Taken together, our liver proteomics may reveal a distinct metabolic transition from fatty acids beta-oxidation in obese monkey to BCAA degradation in diabetic monkeys. |
| WOS关键词 | NONALCOHOLIC-FATTY-LIVER ; HEPATIC INSULIN-RESISTANCE ; OXIDATIVE-PHOSPHORYLATION ; PROTEIN-KINASE ; SUPER-SILAC ; MITOCHONDRIAL DYSFUNCTION ; FASTING HYPERGLYCEMIA ; MACACA-MULATTA ; DISEASE ; ASSOCIATION |
| 资助项目 | National Natural Science Foundation of China[U1402225] ; National Natural Science Foundation of China[U1702288] ; National Natural Science Foundation of China[31671230] ; National Natural Science Foundation of China[31460268] ; National Natural Science Foundation of China[81700520] ; Chinese Academy of Sciences[XDB13030600] ; Yunnan Natural Science Foundation[2017FA007] ; Yunnan Provincial Science and Technology Department[2014HB022] ; Yunnan Oversea High-Level Talent Program[2015HA039] ; Yunnan Oversea High-Level Talent Program[2015HA040] |
| WOS研究方向 | Endocrinology & Metabolism ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000441173000017 |
| 出版者 | AMER PHYSIOLOGICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279642]  |
| 专题 | 分析化学研究室 |
| 通讯作者 | Liang, Bin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China; 2.Soochow Univ, Coll Pharmaceut Sci, Suzhou, Peoples R China; 3.Chinese Acad Sci, Chinese Acad Sci & Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming, Yunnan, Peoples R China; 4.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromolecules, Beijing, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Materia Med, Dept Analyt Chem, Shanghai, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Materia Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China; 7.Kuruning Med Univ, Yanan Affiliated Hosp, Dept Geriatr, Key Lab Cardiovasc Dis Yunnan Prov, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Junlong,Xu, Shimeng,Gao, Jing,et al. SILAC-based quantitative proteomic analysis of the livers of spontaneous obese and diabetic rhesus monkeys[J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,2018,315(2):E294-E306. |
| APA | Wang, Junlong.,Xu, Shimeng.,Gao, Jing.,Zhang, Linqiang.,Zhang, Zhiguo.,...&Liang, Bin.(2018).SILAC-based quantitative proteomic analysis of the livers of spontaneous obese and diabetic rhesus monkeys.AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM,315(2),E294-E306. |
| MLA | Wang, Junlong,et al."SILAC-based quantitative proteomic analysis of the livers of spontaneous obese and diabetic rhesus monkeys".AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 315.2(2018):E294-E306. |
入库方式： OAI收割
来源：上海药物研究所
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