A binding-block ion selective mechanism revealed by a Na/K selective channel
文献类型：期刊论文
| 作者 | Yu, Jie6; Zhang, Bing1,2,4; Zhang, Yixiao6; Xu, Cong-qiao3,5; Zhuo, Wei6; Ge, Jingpeng6; Li, Jun3,5 ; Gao, Ning6; Li, Yang4; Yang, Maojun6
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| 刊名 | PROTEIN & CELL
 |
| 出版日期 | 2018-07 |
| 卷号 | 9期号:7页码:629-639 |
| 关键词 | cryo-EM MscS Na+/K+ selective channel |
| ISSN号 | 1674-800X |
| DOI | 10.1007/s13238-017-0465-8 |
| 文献子类 | Article |
| 英文摘要 | Mechanosensitive (MS) channels are extensively studied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of channel gating and ion selectivity are largely unknown. Here, we identified the YnaI channel as the Na+/K+ cation-selective MS channel and solved its structure at 3.8 by cryo-EM single-particle method. YnaI exhibits low conductance among the family of MS channels in E. coli, and shares a similar overall heptamer structure fold with previously studied MscS channels. By combining structural based mutagenesis, quantum mechanical and electrophysiological characterizations, we revealed that ion selective filter formed by seven hydrophobic methionine (YnaI(Met158)) in the transmembrane pore determined ion selectivity, and both ion selectivity and gating of YnaI channel were affected by accompanying anions in solution. Further quantum simulation and functional validation support that the distinct binding energies with various anions to YnaI(Met158) facilitate Na+/K+ pass through, which was defined as binding-block mechanism. Our structural and functional studies provided a new perspective for understanding the mechanism of how MS channels select ions driven by mechanical force. |
| WOS关键词 | GATED SODIUM-CHANNEL ; CRYOELECTRON MICROSCOPY STRUCTURE ; CRYSTAL-STRUCTURE ; MECHANOSENSITIVE CHANNEL ; K+ CHANNEL ; CRYO-EM ; RESOLUTION ; COMPLEX ; MSCS ; ARCHITECTURE |
| 资助项目 | Ministry of Science and Technology[2016YFA0501100] ; Ministry of Science and Technology[2017YFA0504600] ; Ministry of Science and Technology[2016YFA0500700] ; Ministry of Science and Technology[2013CB910400] ; National Fund for Distinguished Young Scholar[31625008] ; National Natural Science Foundation of China[21532004] ; National Natural Science Foundation of China[31570733] ; National Natural Science Foundation of China[31422016] ; National Natural Science Foundation of China[31371066] ; National Natural Science Foundation of China[31671049] ; National Natural Science Foundation of China[91426302] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6321830 |
| WOS记录号 | WOS:000436455300004 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279687]  |
| 专题 | 药理学第二研究室 |
| 通讯作者 | Gao, Ning; Li, Yang; Yang, Maojun |
| 作者单位 | 1.Tongji Univ, Sch Med, Shanghai Matern & Infant Hosp 1, Anesthesiol, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Tsinghua Univ, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China; 5.Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China; 6.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Minist Educ, Key Lab Prot Sci,Sch Life Sci,Tsinghua Peking Joi, Beijing 100084, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yu, Jie,Zhang, Bing,Zhang, Yixiao,et al. A binding-block ion selective mechanism revealed by a Na/K selective channel[J]. PROTEIN & CELL,2018,9(7):629-639. |
| APA | Yu, Jie.,Zhang, Bing.,Zhang, Yixiao.,Xu, Cong-qiao.,Zhuo, Wei.,...&Yang, Maojun.(2018).A binding-block ion selective mechanism revealed by a Na/K selective channel.PROTEIN & CELL,9(7),629-639. |
| MLA | Yu, Jie,et al."A binding-block ion selective mechanism revealed by a Na/K selective channel".PROTEIN & CELL 9.7(2018):629-639. |
入库方式： OAI收割
来源：上海药物研究所
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