Purification and Analysis of Male Germ Cells from Adult Mouse Testis
文献类型：期刊论文
| 作者 | Buchou, Thierry4; Tan, Minjia1 ; Barral, Sophie3; Vitte, Anne-Laure3; Rousseaux, Sophie3; Arechaga, Juan2; Khochbin, Saadi3
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| 刊名 | Methods in molecular biology (Clifton, N.J.)
 |
| 出版日期 | 2017 |
| 卷号 | 1510页码:159-168 |
| ISSN号 | 1940-6029 |
| DOI | 10.1007/978-1-4939-6527-4_12 |
| 文献子类 | Article |
| 英文摘要 | Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the fractionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266814]  |
| 专题 | 化学蛋白质组学研究中心 |
| 通讯作者 | Buchou, Thierry |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; 2.Laboratory of Stem Cells, Development and Cancer, Department of Cell Biology and Histology, University of the Basque Country (UPV/EHU), Vizcaya, Spain 3.CNRS UMR 5209, INSERM U1209, CR UGA, Institute for Advanced Biosciences, La Tronche, 38700, France; 4.CNRS UMR 5209, INSERM U1209, CR UGA, Institute for Advanced Biosciences, La Tronche, 38700, France. thierry; |
| 推荐引用方式 GB/T 7714 | Buchou, Thierry,Tan, Minjia,Barral, Sophie,et al. Purification and Analysis of Male Germ Cells from Adult Mouse Testis[J]. Methods in molecular biology (Clifton, N.J.),2017,1510:159-168. |
| APA | Buchou, Thierry.,Tan, Minjia.,Barral, Sophie.,Vitte, Anne-Laure.,Rousseaux, Sophie.,...&Khochbin, Saadi.(2017).Purification and Analysis of Male Germ Cells from Adult Mouse Testis.Methods in molecular biology (Clifton, N.J.),1510,159-168. |
| MLA | Buchou, Thierry,et al."Purification and Analysis of Male Germ Cells from Adult Mouse Testis".Methods in molecular biology (Clifton, N.J.) 1510(2017):159-168. |
入库方式： OAI收割
来源：上海药物研究所
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