Bioactivity and Mechanism Study of Waltonitone on Tumor Cells Growth in vitro
文献类型：期刊论文
| 作者 | ZHANG Zhang2; DUAN Chao-hui2; DING Kan1 ; WANG Zheng-tao2
|
| 刊名 | Chinese Pharmaceutical Journal
 |
| 出版日期 | 2010 |
| 卷号 | 45期号:4页码:259-263 |
| 关键词 | ERK1/2 p53 waltonitone tumor cell cycle ERK1/2 p53 heparanase |
| ISSN号 | 1001-2494 |
| 其他题名 | 长梗秦艽酮体外抗肿瘤活性及其作用机制探讨 |
| 文献子类 | Article |
| 英文摘要 | OBJECTIVE To investigate inhibitory effects of waltonitune (WT), a new ursane-type pentacyclic triterpene isolated from Gentian waltonii Burkill, on several human tumor cells growth in vitro and to understand the possible mechanisms. METHODS The potential effect of WT on the cell viability was examined using MTT assay. The cell cycle distribution of BEL7402 cells treated with WT was analyzed by flow cytometry. Phosphorylatiun of ERK1/2 and p38 MAPK were detected by Western blot, while the mRNA ex-pression of p53 and heparanase were determined by RT-PCR analysis. RESULTS WT strongly inhibited the BEL7402, HeLa, BX-PC-3, PANC-1 cells growth. WT induced S phase cell cycle arrest and activated ERK1/2 phosphorylation in BEL-7402 cells. The mR-NA expression of p53 was up-regulated during WT treatment. However, the mRNA expression of heparanase was significantly de-creased. CONCLUSION The anti-tumor activity of WT might be attributed partly to the induction of cell cycle arrest through ERK1/ 2 pathway and inhibition of heparanase expression by up-regulatian of p53 gene. |
| 资助项目 | 国家自然科学基金[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3814784 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267930]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | DING Kan |
| 作者单位 | 1.Glycobiology and Glycochemistry laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | ZHANG Zhang,DUAN Chao-hui,DING Kan,et al. Bioactivity and Mechanism Study of Waltonitone on Tumor Cells Growth in vitro[J]. Chinese Pharmaceutical Journal,2010,45(4):259-263. |
| APA | ZHANG Zhang,DUAN Chao-hui,DING Kan,&WANG Zheng-tao.(2010).Bioactivity and Mechanism Study of Waltonitone on Tumor Cells Growth in vitro.Chinese Pharmaceutical Journal,45(4),259-263. |
| MLA | ZHANG Zhang,et al."Bioactivity and Mechanism Study of Waltonitone on Tumor Cells Growth in vitro".Chinese Pharmaceutical Journal 45.4(2010):259-263. |
入库方式： OAI收割
来源：上海药物研究所
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