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Cell Protection of Polysaccharide from Ophiopogon Japonieus under the Condition of Oxygen Glucose Deprivation through SIPI Signaling Pathway

文献类型：期刊论文

作者WANG Shu1; FENG YI1; DING Kan3; WANG Xin-ping1; XU De-sheng2
刊名Pharmaceutical Biotechnology
出版日期2009
卷号16期号:3页码:220-224
关键词siRNA Ophiopogon japonicus Polysaccharide SIP OGD Eukaryotic expression vector siRNA
ISSN号1005-8915
其他题名麦冬多糖通过S1P1通路保护OGD条件下细胞生存
文献子类Article
英文摘要To study the cytoprotective effect of a polysaccharide from Ophiopogon japonicus(MDG-1)and the possible mechanism involved with S1P1 signal pathway.The S1P1 signaling pathways involved in the cytoprotective effect of MDG-1 was examined by RT-PCR and Western blotting.Blocking the S1P1 gene by chemical synthesized siRNA and constructing the eukaryotic expression vectors containing the coding region of human full length S1P1 gene as a positive control.The cell protective effect and mechanism of MDG-1 were measured by Oxygen Glucose Deprivation(OGD)induced ischemia.The results showed that MDG-1 could up-regulate the expression of S1P1 in mRNA and protein level.MDG-1 could remarkably prevent cells from death induced by OGD and this effect was abolished when cells were transfected with S1P1 siRNA.Meanwhile,the results of the restriction enzyme digestion,PCR and sequencing demonstrated that the vectors were successfully constructed and could also prevent cells from death induced by OGD when cells were transfected with S1P1-pcDNA3.1b vector.It can be concluded that MDG- 1 probably protects cells from death induced by OGD through S1P1 signal pathway.
资助项目上海市教委重点学科[00000000]
WOS研究方向Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:3541383
源URL[http://119.78.100.183/handle/2S10ELR8/268028]  
专题药理学第三研究室
通讯作者DING Kan
作者单位1.Engineering Research Center of Modern Preparation Technigue of TCM,Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.;
2.Shuguang Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China.
3.Shanghai Institute of Materia Medica ,Chinese Academy of Sciences, Shanghai 201203, China.;
推荐引用方式
GB/T 7714		 WANG Shu,FENG YI,DING Kan,et al. Cell Protection of Polysaccharide from Ophiopogon Japonieus under the Condition of Oxygen Glucose Deprivation through SIPI Signaling Pathway[J]. Pharmaceutical Biotechnology,2009,16(3):220-224.
APA WANG Shu,FENG YI,DING Kan,WANG Xin-ping,&XU De-sheng.(2009).Cell Protection of Polysaccharide from Ophiopogon Japonieus under the Condition of Oxygen Glucose Deprivation through SIPI Signaling Pathway.Pharmaceutical Biotechnology,16(3),220-224.
MLA WANG Shu,et al."Cell Protection of Polysaccharide from Ophiopogon Japonieus under the Condition of Oxygen Glucose Deprivation through SIPI Signaling Pathway".Pharmaceutical Biotechnology 16.3(2009):220-224.

入库方式： OAI收割

来源：上海药物研究所

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