热门
Isolation, purification and structural analysis of a polysaccharide MDG-1 from Ophiopogon japonicus
文献类型：期刊论文
| 作者 | Xu Desheng1; Feng Yi1; Lin Xiao1; Deng Hailin1; Fang Jinian2; Dong Qun2 |
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2005 |
| 卷号 | 40期号:7页码:636-639 |
| 关键词 | Ophiopogon japonicus polysaccharide structure |
| ISSN号 | 0513-4870 |
| 其他题名 | 麦冬多糖MDG-1的分离纯化和结构分析 |
| 文献子类 | Article |
| 英文摘要 | Aim To separate and purify the anti-myocardial ischemic polysaccharide fraction with a homogenous molecular weight from Ophiopogon japonicus, then study the chemical structure of the parts. Methods Crude polysaccharides were prepared by extracting the tube root fraction of Ophiopogon japonicus with water, then precipitation with ethanol. From the crude polysaccharides, the polysaccharide of MDG-1 was separated and purified using ultrafiltration, DEAE Sepharose FF and Sephadex G-25 column ehromatography. Its structure was studied by complete hydrolysis, periodate oxidation, Smith degradation, methylation analysis, 'H NMR and 13C NMR analysis etc. Results and Conclusion MDG-1 was a water-soluble beta-D-fructosan, containing a backbone composed of Fruf (21), and a branch of Fruf (26) Fruf (2 per average 2. 8 of main chain residues. Mn, Mw and Mp of MDG-1 were 3 400, 4 800 and 5 000, respectively. MDG-1 contains trace of Glc, which maybe connect to its reducing terminal. Molar ratio of Fru and Glc is approximately 35: 1. |
| 资助项目 | 国家高技术研究发展计划[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2135161 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268355]  |
| 专题 | 药理学第三研究室 |
| 作者单位 | 1.Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.; 2.Shanghai Institute of Materia Median, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Xu Desheng,Feng Yi,Lin Xiao,et al. Isolation, purification and structural analysis of a polysaccharide MDG-1 from Ophiopogon japonicus[J]. Acta Pharmaceutica Sinica,2005,40(7):636-639. |
| APA | Xu Desheng,Feng Yi,Lin Xiao,Deng Hailin,Fang Jinian,&Dong Qun.(2005).Isolation, purification and structural analysis of a polysaccharide MDG-1 from Ophiopogon japonicus.Acta Pharmaceutica Sinica,40(7),636-639. |
| MLA | Xu Desheng,et al."Isolation, purification and structural analysis of a polysaccharide MDG-1 from Ophiopogon japonicus".Acta Pharmaceutica Sinica 40.7(2005):636-639. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。