人细胞周期蛋白D1/CDK4基因的真核表达及生物活性鉴定
文献类型：期刊论文
| 作者 | Wang Honghe1; Zhu Xiaofeng1; Xie Bingfen1; Feng Gongkan1; Shen Jingkang2; Liu Zongchao1 |
| 刊名 | Chinese Journal of Biochemistry and Molecular Biology
 |
| 出版日期 | 2003 |
| 卷号 | 19期号:6页码:709-714 |
| 关键词 | CDK4 cyclin D1 CDK4 baculovirus expression system molecular target |
| ISSN号 | 1007-7626 |
| 文献子类 | Article |
| 英文摘要 | Cyclin Dl and CDK4 fusion-proteins were used in antiproliferative inhibitor screening. Cyclin Dl and CDK4 genes were obtained by RT-PCR from HL-60 cells and inserted into pGEM-T Easy vector. The genes were released from T-vector by endonuclease and insered into insect baculovirus donor plasmid. Recombinant vectors were identified by restriction enzyme cutting and homologues recombination in E coli DHlOBac. Recombinant bacmid DNA was transformed into insect cell Tn-5Bl-4. The proteins were identified by SDS-PAGE and Western blotting. The activity of Cyclin D1/CDK4 was detected by phosphorylation of GST-Rb in kinase assay. The insect expressing vectors could express Cyclin Dl and CDK4 proteins which possessed ability of phosphorylating Rb protein, showing biological activity. Cyclin Dl and CDK4 proteins were expressed correctly in insect cells and had their biological activity. |
| 资助项目 | 国家自然科学基金[00000000] ; 国家863计划[00000000] ; 中山医科大学"211工程"肿瘤学重点学科基金[00000000] |
| WOS研究方向 | Genetics & Heredity (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1324409 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268435]  |
| 专题 | 药物化学研究室 |
| 作者单位 | 1.Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, China.; 2.Shanghai Institute of Materia Medical, Chinese Academy of Sciences, Shanghai 200031, China. |
| 推荐引用方式 GB/T 7714 | Wang Honghe,Zhu Xiaofeng,Xie Bingfen,等. 人细胞周期蛋白D1/CDK4基因的真核表达及生物活性鉴定[J]. Chinese Journal of Biochemistry and Molecular Biology,2003,19(6):709-714. |
| APA | Wang Honghe,Zhu Xiaofeng,Xie Bingfen,Feng Gongkan,Shen Jingkang,&Liu Zongchao.(2003).人细胞周期蛋白D1/CDK4基因的真核表达及生物活性鉴定.Chinese Journal of Biochemistry and Molecular Biology,19(6),709-714. |
| MLA | Wang Honghe,et al."人细胞周期蛋白D1/CDK4基因的真核表达及生物活性鉴定".Chinese Journal of Biochemistry and Molecular Biology 19.6(2003):709-714. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。