Comparison of reliabilities of mass spectrometry-based label-free quantitation methods for histone post-translational modification analysis
文献类型：期刊论文
| 作者 | Liu Zhiwei2; Zhu Mingrui2; Zhai Linhui1; Tan Minjia2
|
| 刊名 | Chinese Journal of Chromatography
 |
| 出版日期 | 2016 |
| 卷号 | 34期号:9页码:825-830 |
| 关键词 | histone post-translational modification label-free quantitation reliability |
| ISSN号 | 1000-8713 |
| 其他题名 | 组蛋白翻译后修饰无标定量方法可靠性的比较 |
| 文献子类 | Article |
| 英文摘要 | Histone post-translational modifications (PTMs) play critical roles in epigenetic regulations of cellular physiology. The rapid development of mass spectrometry based on proteomics makes it possible to systematically widely identify and quantify the dynamic changes of histone PTMs. There are three widely used label-free quantitation methods (spectra count, peak area and intensity) for mass spectrometry data analysis, but by which method is the most reliable one for histone PTM quantification has not been systematically investigated. To this goal, we quantified histone acetylome in stable isotope amino acid labeled SH-SY5Y cells by SAHA (a pan histone deacetylase inhibitor) treatment. By comparing the standard deviations (SDs) of quantitation results from the three methods, we showed that quantification based on peak area method is the most reliable one. Therefore, our study provided new insight for label-free histone mark analysis, especially for clinical and large-scale samples. |
| 资助项目 | 上海市科学技术委员会项目[00000000] ; 国家自然科学基金项目[00000000] |
| WOS研究方向 | Chemistry (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5790220 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269238]  |
| 专题 | 化学蛋白质组学研究中心 |
| 通讯作者 | Tan Minjia |
| 作者单位 | 1.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Shanghai 201203, China. 2.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | Liu Zhiwei,Zhu Mingrui,Zhai Linhui,et al. Comparison of reliabilities of mass spectrometry-based label-free quantitation methods for histone post-translational modification analysis[J]. Chinese Journal of Chromatography,2016,34(9):825-830. |
| APA | Liu Zhiwei,Zhu Mingrui,Zhai Linhui,&Tan Minjia.(2016).Comparison of reliabilities of mass spectrometry-based label-free quantitation methods for histone post-translational modification analysis.Chinese Journal of Chromatography,34(9),825-830. |
| MLA | Liu Zhiwei,et al."Comparison of reliabilities of mass spectrometry-based label-free quantitation methods for histone post-translational modification analysis".Chinese Journal of Chromatography 34.9(2016):825-830. |
入库方式： OAI收割
来源：上海药物研究所
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