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Structure features of polysaccharides from Chrysanthemum morifolium and their activities against tumor cells and NF-kappaB

文献类型：期刊论文

作者Fan Lingjing2; Ni Xinyan3; Wu Chunjie1; Ding Kan3
刊名Chinese Traditional and Herbal Drugs
出版日期2013
卷号44期号:17页码:2364-2371
关键词NF-kappaB Chrysanthemum morifolium Ramat. neutral polysaccharide PANC-1 cell LO2 cell NF-kappaB
ISSN号0253-2670
其他题名菊花多糖的结构特征及其对NF-kappaB和肿瘤细胞的活性研究
文献子类Article
英文摘要Objective To clarify the structure features of polysaccharides from Chrysanthemum morifolium (PCM) and to study their activities against tumor cells and NF-kappaB. Methods Six homogeneous neutral polysaccharides were obtained from three kinds of C. morifolium (Hangju, Huaiju, and Boju) flowers by successive hot water extraction, followed by ethanol precipitation, ion-exchange chromatography, and gel permeation chromatography. Their primary structures were characterized by HPGPC, IR, GC, and GC-MS analyses. Their bioactivities were examined by MTT assay using PANC-1 and LO2 cells. In addition, NF-kappaB signaling activation in PANC-1 and LO2 cells treated by polysaccharides were also measured. Results The weight-average molecular mass of the six PCM, CMTA0S1, CMTA0S3, CMJA0S1, CMJA0S2, CMBA0S1, and CMBA0S3 was 7.523 * 10~4, 7.80 * 10~3, 7.80 * 10~4, 1.04 * 10~4, 5.79 * 10~4, and 1.35 * 10~4, respectively. CMTA0S1, CMJA0S1, and CMBA0S1 mainly contained galactose (Gal), arabinose (Ara) and glucose (Glc) residues in molar ratio of 1.23∶1.00∶0.20, 2.18∶1.00∶0.53, and 3.30∶1.00∶0.75, while CMTA0S3, CMJA0S2, and CMBA0S3 mainly contained Gal, Ara, Glc, and mannose (Man) residues in molar ratio of 0.73∶1.00∶0.40∶0.21, 1.39∶1.00∶0.84∶0.55, and 1.19∶1.00∶0.48∶0.19. Methylation analysis indicated that six PCM primarily consisted of T-arabinofuranosyl, 1, 5-arabinofuranosyl, 1, 4-galactopyranosyl, 1, 3, 6-galactopyranosyl, and 1, 4-glucopyranosyl residues. The biological activity study suggested that all the PCM could inhibit the growth of PANC-1 cells. Among them the inhibitory rates of CMTA0S3 and CMJA0S2 were at most to 70% with concentration-effect relationship. The NF-kappaB inhibition test indicated that only the crude polysaccharide CMBA had strong immunosuppressive activity, and homogeneous polysaccharides CMTA0S1 and CMJA0S1 showed potential immunostimulation. Conclusion The six homogeneous polysaccharides share similar structures and inhibition on PANC-1 cells growth. Meanwhile they also may regulate the NF-kappaB activation.
资助项目国家杰出青年科学基金资助项目[00000000]
WOS研究方向General & Internal Medicine (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:4943619
源URL[http://119.78.100.183/handle/2S10ELR8/269370]  
专题药理学第三研究室
通讯作者Ding Kan
作者单位1.Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China.
2.Chengdu University of Traditional Chinese Medicine, Glycochemistry & Glycobiology Laboratory, Chengdu, Sichuan 611137, China.;
3.Glycochemistry & Glycobiology Laboratory, Shanghai Institutes of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.;
推荐引用方式
GB/T 7714		 Fan Lingjing,Ni Xinyan,Wu Chunjie,et al. Structure features of polysaccharides from Chrysanthemum morifolium and their activities against tumor cells and NF-kappaB[J]. Chinese Traditional and Herbal Drugs,2013,44(17):2364-2371.
APA Fan Lingjing,Ni Xinyan,Wu Chunjie,&Ding Kan.(2013).Structure features of polysaccharides from Chrysanthemum morifolium and their activities against tumor cells and NF-kappaB.Chinese Traditional and Herbal Drugs,44(17),2364-2371.
MLA Fan Lingjing,et al."Structure features of polysaccharides from Chrysanthemum morifolium and their activities against tumor cells and NF-kappaB".Chinese Traditional and Herbal Drugs 44.17(2013):2364-2371.

入库方式： OAI收割

来源：上海药物研究所

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