Leucine-2-alanine enkephalin induced delta opioid receptors internalization expressed stably in CHO cells
文献类型：期刊论文
| 作者 | Wang, CH ; Zhou, DH; Chen, J; Chi, ZQ
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 1999-06 |
| 卷号 | 20期号:6页码:491-494 |
| 关键词 | leucine-2-alanine enkephalin CHO cells radioligand assay delta opioid receptors fluorescence microscopy diprenorphine pertussis toxins staurosporine |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To characterize the internalization of delta opioid receptors (DOR) stably expressed in Chinese hamster ovary (CHO) cells and the role of the C-terminal in this process. METHODS: Receptor membrane anchoring was shown by immunofluorescence microscopy. Receptor internalization was assessed by measuring the radioligand binding resistant to the acid-buffer wash. RESULTS: Originally, all the wildtype (CHO-W) and C-truncated (CHO-T) DOR expressed were localized to the membrane. Agonist [H-3] leucine3-alanine enkephalin (LAE) but not the antagonist [3H]diprenorphine (Dip) induced rapid receptor internalization. The internalization of C-truncated DOR in CHO-T was similar to that of the wild-type in maximal level, but climbed up more slowly. DOR internalization was extracellular osmolarity- and temperature-sensitive. Pertussis toxin and universal protein kinase inhibitor staurosporine had no effect on it. CONCLUSION: DOR internalization is an agonist and clathrin-coated pits dependent, but post-receptor cellular signal transduction independent process; moreover, the C-terminal of DOR, not engaged in membrane anchoring, affects the initialization of DOR internalization. |
| WOS关键词 | DYNAMIN-DEPENDENT ENDOCYTOSIS ; MEDIATED INTERNALIZATION ; AGONIST ; DESENSITIZATION ; ACTIVATION ; TERMINUS ; SERINE ; DOMAIN ; KAPPA |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000080905900003 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274720]  |
| 专题 | 生物技术药物研发中心（筹） |
| 通讯作者 | Zhou, DH |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, CH,Zhou, DH,Chen, J,et al. Leucine-2-alanine enkephalin induced delta opioid receptors internalization expressed stably in CHO cells[J]. ACTA PHARMACOLOGICA SINICA,1999,20(6):491-494. |
| APA | Wang, CH,Zhou, DH,Chen, J,&Chi, ZQ.(1999).Leucine-2-alanine enkephalin induced delta opioid receptors internalization expressed stably in CHO cells.ACTA PHARMACOLOGICA SINICA,20(6),491-494. |
| MLA | Wang, CH,et al."Leucine-2-alanine enkephalin induced delta opioid receptors internalization expressed stably in CHO cells".ACTA PHARMACOLOGICA SINICA 20.6(1999):491-494. |
入库方式： OAI收割
来源：上海药物研究所
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