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Ginsenoside Rb2 enhances the anti-inflammatory effect of omega-3 fatty acid in LPS-stimulated RAW264.7 macrophages by upregulating GPR120 expression

文献类型：期刊论文

作者Huang, Qi1,2; Wang, Ting1; Wang, He-yao1
刊名ACTA PHARMACOLOGICA SINICA
出版日期2017-02
卷号38期号:2页码:192-200
关键词ginsenoside Rb2 LPS alpha-linolenic acid RAW264.7 cells GPR120 inflammatory cytokines iNOS COX-2 IKK/NF-kappa B MAPK pathway diabetes chronic inflammation
ISSN号1671-4083
DOI10.1038/aps.2016.135
文献子类Article
英文摘要Recent studies confirm that chronic low-grade inflammation is closely associated with metabolic syndromes, and anti-inflammatory therapy is a potential approach for treating cardiovascular diseases and type 2 diabetes. Accumulating evidence suggests that GPR120 activation is a feasible solution to ameliorating chronic inflammation and improving glucose metabolism. In this study we investigated whether ginsenoside Rb2 (Rb2), which exhibited regulatory activities in glucose and lipid metabolism, affected GPR120 expression in lipopolysaccharide (LPS)-activated mouse macrophage RAW264.7 cells, and examined the contribution of GPR120 activation to reducing the LPS-induced inflammatory response. LPS (100 ng/mL) activated the macrophages, resulting in dramatic increases in TNF-alpha, IL-6, IL-1 beta and NO production. Treatment with a omega-3 fatty acid alpha-linolenic acid (ALA, 50 mu mol/L) produced moderate reduction in LPS-stimulated inflammatory cytokines and NO production (TNF-alpha and IL-6 were decreased by 46% and 42%, respectively). Pre-incubation with Rb2 (1 or 10 mu mol/L) for 12 h before ALA treatment dramatically amplified the inhibitory effects of ALA (TNF-alpha and IL-6 were decreased by 74% and 86%, respectively). Compared to the treatment with ALA alone, pre-incubation with Rb2 resulted in a more prominent reduction in LPS-stimulated expression of iNOS and COX-2 and LPS-stimulated IKK/NF-kappa B phosphorylation and MAPK pathway activation. Rb2 (0.1-100 mu mol/L) dose- and time-dependently increased both mRNA and protein expression of GPR120 in RAW264.7 cells, but treatment with Rb2 alone did not exert anti-inflammatory effect in LPS-activated RAW264.7 cells. In RAW264.7 cells transfected with GPR120 shRNA, the ameliorating effects of Rb2 on LPS-induced inflammation were abolished. In conclusion, Rb2 exerts anti-inflammatory effect in LPS-stimulated mouse macrophage RAW264.7 cells in vitro by increasing GPR120 expression and subsequently enhancing omega-3 fatty acid-induced GPR120 activation.
WOS关键词INSULIN-RESISTANCE ; FATTY-ACIDS ; CYCLOOXYGENASE-2 INDUCTION ; DOCOSAHEXAENOIC ACID ; INDUCED APOPTOSIS ; ADIPOSE-TISSUE ; CELLS ; ACTIVATION ; INFLAMMATION ; POLARIZATION
资助项目National Natural Science Foundation[81473262] ; National Natural Science Foundation[81503124] ; Science and Technology Commission of Shanghai Municipality[15431901000] ; Science and Technology Commission of Shanghai Municipality[14ZR1447700] ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences[CASIMM0120162025] ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences[CASIMM0120164014]
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:5906829
WOS记录号WOS:000393013400004
出版者ACTA PHARMACOLOGICA SINICA
源URL[http://119.78.100.183/handle/2S10ELR8/275659]  
专题药理学第三研究室
通讯作者Wang, He-yao
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Qi,Wang, Ting,Wang, He-yao. Ginsenoside Rb2 enhances the anti-inflammatory effect of omega-3 fatty acid in LPS-stimulated RAW264.7 macrophages by upregulating GPR120 expression[J]. ACTA PHARMACOLOGICA SINICA,2017,38(2):192-200.
APA Huang, Qi,Wang, Ting,&Wang, He-yao.(2017).Ginsenoside Rb2 enhances the anti-inflammatory effect of omega-3 fatty acid in LPS-stimulated RAW264.7 macrophages by upregulating GPR120 expression.ACTA PHARMACOLOGICA SINICA,38(2),192-200.
MLA Huang, Qi,et al."Ginsenoside Rb2 enhances the anti-inflammatory effect of omega-3 fatty acid in LPS-stimulated RAW264.7 macrophages by upregulating GPR120 expression".ACTA PHARMACOLOGICA SINICA 38.2(2017):192-200.

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来源：上海药物研究所

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