Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity
文献类型：期刊论文
| 作者 | Lin, Liyan1,2; Wang, Peipei1 ; Du, Zhenyun1; Wang, Wucheng1; Cong, Qifei1; Zheng, Changping1; Jin, Can1,2; Ding, Kan1,2; Shao, Chenghao3
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2016-07 |
| 卷号 | 88页码:130-137 |
| 关键词 | Lonicera japonica Polysaccharide Antipancreatic cancer cells growth |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2016.03.025 |
| 文献子类 | Article |
| 英文摘要 | To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L.japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54 kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2 M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked beta-D-Galp, T- and 1,5-linked alpha-L-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1 mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | CHRYSANTHEMUM-MORIFOLIUM FLOWERS ; RHAMNOGALACTURONAN I ; CELL-PROLIFERATION ; POLYSACCHARIDE ; ARABINOGALACTAN |
| 资助项目 | State Key Program of National Natural Science Foundation of China[31230022] ; New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; Natural Science Foundation of China[31400690] ; National Science Fund for Distinguished Young Scholars in China[81125025] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000376800600016 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275980]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Ding, Kan; Shao, Chenghao |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 2.Zunyi Med Univ, Sch Pharm, 201 Dalian Rd, Zunyi 563003, Peoples R China; 3.Shanghai Changzheng Hosp, Dept Gen Surg, 415 Fengyang Rd, Shanghai 200003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Liyan,Wang, Peipei,Du, Zhenyun,et al. Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,88:130-137. |
| APA | Lin, Liyan.,Wang, Peipei.,Du, Zhenyun.,Wang, Wucheng.,Cong, Qifei.,...&Shao, Chenghao.(2016).Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,88,130-137. |
| MLA | Lin, Liyan,et al."Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 88(2016):130-137. |
入库方式： OAI收割
来源：上海药物研究所
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