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The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa

文献类型：期刊论文

作者Yang, Nana3; Ding, Shuting2,3; Chen, Feifei3; Zhang, Xue3; Xia, Yongjie3; Di, Hongxia3; Cao, Qiao3; Deng, Xin1,6; Wu, Min4; Wong, Catherine C. L.5
刊名MOLECULAR MICROBIOLOGY
出版日期2015-05
卷号96期号:3页码:526-547
ISSN号0950-382X
DOI10.1111/mmi.12954
文献子类Article
英文摘要Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P.aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruption of the gene encoding the Lon protease entirely offsets the effect of crc deletion on the production of both rhamnolipid and rhlQS signal C4-HSL. Crc is unable to bind lon mRNAin vitro in the absence of the RNA chaperon Hfq, while Crc contributes to Hfq-mediated repression of the lon gene expression at a posttranscriptional level. Deletion of crc, which results in up-regulation of lon, significantly reduces the in vivo stability and abundance of the RhlI protein that synthesizes C4-HSL, causing the attenuation of rhlQS. Lon is also capable of degrading the RhlI protein in vitro. In addition, constitutive expression of rhlI suppresses the defects of the crc deletion mutant in rhamnolipid, C4-HSL and virulence on lettuce leaves. This study therefore uncovers a novel posttranscriptional regulatory cascade, Crc-Hfq/Lon/RhlI, for the regulation of rhamnolipid production and rhlQS in P.aeruginosa.
WOS关键词CARBON CATABOLITE REPRESSION ; ESCHERICHIA-COLI ; GLOBAL REGULATOR ; BIOSURFACTANT SYNTHESIS ; STAPHYLOCOCCUS-AUREUS ; BACTERIAL VIRULENCE ; BIOFILM FORMATION ; QUALITY-CONTROL ; RNA-SEQ ; EXPRESSION
资助项目National Natural Science Foundation of China[31270126] ; Shanghai Committee of Science and Technology[12JC1410200] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program'[2014ZX09507009-015] ; Chinese Academy of Sciences[00000000]
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
语种英语
WOS记录号WOS:000353895800007
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/276556]  
专题药理学第三研究室
通讯作者Lan, Lefu
作者单位1.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA;
2.Nanjing Univ, Inst Chem & BioMed Sci, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
4.Univ N Dakota, Dept Basic Sci, Sch Med & Hlth Sci, Grand Forks, ND 58203 USA;
5.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Mass Spectrometry Div, Natl Ctr Prot Sci Shanghai, Shanghai 201210, Peoples R China
6.Univ Chicago, Dept Chem, Chicago, IL 60637 USA;
推荐引用方式
GB/T 7714		 Yang, Nana,Ding, Shuting,Chen, Feifei,et al. The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa[J]. MOLECULAR MICROBIOLOGY,2015,96(3):526-547.
APA Yang, Nana.,Ding, Shuting.,Chen, Feifei.,Zhang, Xue.,Xia, Yongjie.,...&Lan, Lefu.(2015).The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa.MOLECULAR MICROBIOLOGY,96(3),526-547.
MLA Yang, Nana,et al."The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa".MOLECULAR MICROBIOLOGY 96.3(2015):526-547.

入库方式： OAI收割

来源：上海药物研究所

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