A simplified procedure for gram-scale production of sialylglycopeptide (SGP) from egg yolks and subsequent semi-synthesis of Man(3)GlcNAc oxazoline
文献类型：期刊论文
| 作者 | Sun, Bingyang1,2; Bao, Wenzheng2,3; Tian, Xiaobo2; Li, Mingjing2,3; Liu, Hong2 ; Dong, Jinhua1; Huang, Wei2,3
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| 刊名 | CARBOHYDRATE RESEARCH
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| 出版日期 | 2014-09-19 |
| 卷号 | 396页码:62-69 |
| 关键词 | Egg yolk Sialylglycopeptide Man3GlcNAc oxazoline Chemoenzymatic transglycosylation |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2014.07.013 |
| 文献子类 | Article |
| 英文摘要 | Heterogeneity of glycan structures in native glycoconjugates always hampers precise studies on carbohydrate-involved biological functions. To construct homogeneous glycoconjugates from natural resource of homogeneous glycans is therefore a practical approach to solve this problem. We report here an optimized procedure for gram-scale production of sialylglycopeptide (SGP) containing a disialyl biantennary complex-type N-glycan from egg yolks. Our new procedure simplified the extraction process by treating the egg yolk powder with 40% acetone, avoiding massive emulsification, high-speed centrifugation, and sophisticated chromatography in reported methods. Subsequent semi-synthesis of the N-glycan core Man(3)GlcNAc oxazoline from SGP was accomplished for the first-time via glyco-trimming and successive oxazoline formation. This efficient semi-synthesis provides an alternative to the pure chemical approach that involves multi-step total synthesis and facilitates the application of endo-glycosidase-enabled chemoenzymatic synthesis of various homogeneous glycoconjugates. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS关键词 | BETA-N-ACETYLGLUCOSAMINIDASE ; ENDOHEXOSAMINIDASE-CATALYZED GLYCOSYLATION ; COMPLEX-TYPE OLIGOSACCHARIDES ; CHEMOENZYMATIC SYNTHESIS ; MICROBIAL ENDOGLYCOSIDASE ; GLYCOPROTEIN-SYNTHESIS ; SUGAR OXAZOLINES ; RECEPTOR-BINDING ; QUALITY-CONTROL ; GLYCOPEPTIDE |
| 资助项目 | National Natural Science Foundation of China (NNSFC)[21372238] ; National 1000-Young-Talent Program of China[00000000] ; Shanghai Pujiang Talent Program[13PJ1410200] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000341874600009 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276898]  |
| 专题 | 生物技术药物研发中心（筹） 药物化学研究室 药物安全性评价中心 |
| 通讯作者 | Dong, Jinhua |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Minist Educ, Key Lab Struct Based Drug Design & Discovery, Shenyang 110016, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 3.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Bingyang,Bao, Wenzheng,Tian, Xiaobo,et al. A simplified procedure for gram-scale production of sialylglycopeptide (SGP) from egg yolks and subsequent semi-synthesis of Man(3)GlcNAc oxazoline[J]. CARBOHYDRATE RESEARCH,2014,396:62-69. |
| APA | Sun, Bingyang.,Bao, Wenzheng.,Tian, Xiaobo.,Li, Mingjing.,Liu, Hong.,...&Huang, Wei.(2014).A simplified procedure for gram-scale production of sialylglycopeptide (SGP) from egg yolks and subsequent semi-synthesis of Man(3)GlcNAc oxazoline.CARBOHYDRATE RESEARCH,396,62-69. |
| MLA | Sun, Bingyang,et al."A simplified procedure for gram-scale production of sialylglycopeptide (SGP) from egg yolks and subsequent semi-synthesis of Man(3)GlcNAc oxazoline".CARBOHYDRATE RESEARCH 396(2014):62-69. |
入库方式： OAI收割
来源：上海药物研究所
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