Oligosaccharide G19 inhibits U-87 MG human glioma cells growth in vitro and in vivo by targeting epidermal growth factor (EGF) and activating p53/p21 signaling
文献类型：期刊论文
| 作者 | Liu, Hailing2,3; Zhou, Ling2,3; Shi, Songshan1; Wang, Ying3; Ni, Xinyan3; Xiao, Fei3; Wang, Shunchun1; Li, Ping2; Ding, Kan3
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| 刊名 | GLYCOBIOLOGY
 |
| 出版日期 | 2014-08 |
| 卷号 | 24期号:8 |
| 关键词 | apoptosis cell cycle arrest glioma oligosaccharide G19 p53 |
| ISSN号 | 0959-6658 |
| 文献子类 | Article |
| 英文摘要 | G19 is a novel homogeneous sulfated oligosaccharide, prepared from Grateloupia filicina (G. filicina). In the present study, we firstly reported that oligosaccharide G19 exhibited a dose-and time-dependent anti-proliferation effect against U-87 MG human glioma cells. Further studies indicated that G19 strongly bound to epidermal growth factor (EGF), suppressed epidermal growth factor receptor (EGFR) phosphorylation and interrupted the phosphatidylinositol-3 kinase (PI3K)/Akt pathway in the cancer cells. Moreover, G19 elevated intracellular reactive oxygen species (ROS) levels and caused endogenous DNA damage. These actions were associated with activation of ataxia telangiectasia-mutated (ATM)/checkpoint kinase 2 (Chk2) pathway. The down-regulation of MDM2 with stabilizing p53 and the nuclear location of p21 were induced by G19 to cause cell cycle arrest and apoptosis to some extent. Meanwhile, intrinsic mitochondrial pathway and extrinsic death receptor pathway were involved in G19-mediated apoptosis. Pre-treatment with free radical scavenger N-acetyl-L-cysteine (NAC) nearly completely inversed G19-induced cell growth inhibition, cell cycle arrest and apoptosis in U-87 MG cells. Importantly, G19 could inhibit the growth of U-87 MG tumor cells xenograft in nude mice. The results suggested that G19 could be served as a new targeting drug candidate for human glioma treatment. |
| WOS关键词 | NF-KAPPA-B ; DOUBLE-STRAND BREAKS ; CANCER-CELLS ; DNA-DAMAGE ; IONIZING-RADIATION ; TRANSCRIPTIONAL ACTIVATION ; CARCINOMA-CELLS ; FACTOR RECEPTOR ; CYCLE ARREST ; COMET ASSAY |
| 资助项目 | New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; National Science Fund for Distinguished Young Scholars in China[81125025] ; National Natural Science Foundation of China (NSFC)[31230022] ; National Natural Science Foundation of China (NSFC)[81171914] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000339418000008 |
| 出版者 | OXFORD UNIV PRESS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276972]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Ding, Kan |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Key Lab Standardizat Chinese Med, Minist Educ, Shanghai 201203, Peoples R China 2.China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Peoples R China; 3.Chinese Acad Sci, Glycobiol & Glycochem Lab, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, Hailing,Zhou, Ling,Shi, Songshan,et al. Oligosaccharide G19 inhibits U-87 MG human glioma cells growth in vitro and in vivo by targeting epidermal growth factor (EGF) and activating p53/p21 signaling[J]. GLYCOBIOLOGY,2014,24(8). |
| APA | Liu, Hailing.,Zhou, Ling.,Shi, Songshan.,Wang, Ying.,Ni, Xinyan.,...&Ding, Kan.(2014).Oligosaccharide G19 inhibits U-87 MG human glioma cells growth in vitro and in vivo by targeting epidermal growth factor (EGF) and activating p53/p21 signaling.GLYCOBIOLOGY,24(8). |
| MLA | Liu, Hailing,et al."Oligosaccharide G19 inhibits U-87 MG human glioma cells growth in vitro and in vivo by targeting epidermal growth factor (EGF) and activating p53/p21 signaling".GLYCOBIOLOGY 24.8(2014). |
入库方式： OAI收割
来源：上海药物研究所
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