Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa
文献类型：期刊论文
| 作者 | Deng, Xin2,3; Liang, Haihua2,3; Ulanovskaya, Olesya A.1,4; Ji, Quanjiang2,3; Zhou, Tianhong2,3; Sun, Fei2,3; Lu, Zhike2,3; Hutchison, Alan L.2,3; Lan, Lefu5 ; Wu, Min6
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| 刊名 | JOURNAL OF BACTERIOLOGY
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| 出版日期 | 2014-07 |
| 卷号 | 196期号:14页码:2499-2513 |
| ISSN号 | 0021-9193 |
| DOI | 10.1128/JB.01538-14 |
| 文献子类 | Article |
| 英文摘要 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from human pathogens Staphylococcus aureus and Pseudomonas aeruginosa can be readily inhibited by reactive oxygen species (ROS)-mediated direct oxidation of their catalytic active cysteines. Because of the rapid degradation of H2O2 by bacterial catalase, only steady-state but not one-dose treatment with H2O2 rapidly induces glycolysis and the pentose phosphate pathway (PPP). We conducted transcriptome sequencing (RNA-seq) analyses to globally profile the bacterial transcriptomes in response to a steady level of H2O2, which revealed profound transcriptional changes, including the induced expression of glycolytic genes in both bacteria. Our results revealed that the inactivation of GAPDH by H2O2 induces metabolic levels of glycolysis and the PPP; the elevated levels of fructose 1,6-biphosphate (FBP) and 2-keto-3-deoxy-6-phosphogluconate (KDPG) lead to dissociation of their corresponding glycolytic repressors (GapR and HexR, respectively) from their cognate promoters, thus resulting in derepression of the glycolytic genes to overcome H2O2-stalled glycolysis in S. aureus and P. aeruginosa, respectively. Both GapR and HexR may directly sense oxidative stresses, such as menadione. |
| WOS关键词 | OXIDATION-SENSING MECHANISM ; PENTOSE-PHOSPHATE PATHWAY ; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; TRANSCRIPTIONAL REGULATION ; ANTIOXIDANT RESPONSE ; BACILLUS-SUBTILIS ; RNA-SEQ ; STRESS ; REGULATOR ; REDUCTASE |
| 资助项目 | National Institutes of Health[AI074658] ; National Institutes of Health[P50GM081892] ; Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease Award[00000000] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000338765600001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277006]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | He, Chuan |
| 作者单位 | 1.Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA; 2.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 4.Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China; 6.Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58201 USA |
| 推荐引用方式 GB/T 7714 | Deng, Xin,Liang, Haihua,Ulanovskaya, Olesya A.,et al. Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa[J]. JOURNAL OF BACTERIOLOGY,2014,196(14):2499-2513. |
| APA | Deng, Xin.,Liang, Haihua.,Ulanovskaya, Olesya A..,Ji, Quanjiang.,Zhou, Tianhong.,...&He, Chuan.(2014).Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa.JOURNAL OF BACTERIOLOGY,196(14),2499-2513. |
| MLA | Deng, Xin,et al."Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa".JOURNAL OF BACTERIOLOGY 196.14(2014):2499-2513. |
入库方式： OAI收割
来源：上海药物研究所
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