The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of beta-hairpin
文献类型：期刊论文
| 作者 | Chen BaoEn1; Gan JianHua2; Yang CaiGuang1
|
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2014-02 |
| 卷号 | 57期号:2页码:307-313 |
| 关键词 | DNA repair ALKBH2 chemical cross-linking beta-hairpin motif damage detection |
| ISSN号 | 1674-7291 |
| DOI | 10.1007/s11426-013-5029-z |
| 文献子类 | Article |
| 英文摘要 | Mammalian AlkB homologue 2 (ALKBH2) is the primary housekeeping DNA demethylase, effectively repairing endogenously formed methylated lesions in double-stranded DNA. Our previous studies demonstrated that a hydrophobic beta-hairpin motif of ALKBH2 could play crucial roles in base-pair stability interrogation and damaged base flipping. Using chemical cross-linking strategy, we obtained two crystal structures of human ALKBH2 mutant bound to duplex DNA. The structural analysis suggests that the beta-hairpin motif is flexible in conformation and is likely to slide along the DNA duplex in local regions to search for damaged base. This study provides a new mechanistic insight into DNA damage detection by ALKBH2. |
| WOS关键词 | DNA-REPAIR PROTEIN ; ALKYLATION DAMAGE ; ESCHERICHIA-COLI ; DEMETHYLATION ; EXPRESSION ; RESISTANCE ; REVERSAL ; ENZYMES ; LESIONS ; BASES |
| 资助项目 | Hundred Talents Program of the Chinese Academy of Sciences, Key Project of Chinese National Programs for Fundamental Research and Development[2009CB918502] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000330681700013 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277208]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Gan JianHua |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen BaoEn,Gan JianHua,Yang CaiGuang. The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of beta-hairpin[J]. SCIENCE CHINA-CHEMISTRY,2014,57(2):307-313. |
| APA | Chen BaoEn,Gan JianHua,&Yang CaiGuang.(2014).The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of beta-hairpin.SCIENCE CHINA-CHEMISTRY,57(2),307-313. |
| MLA | Chen BaoEn,et al."The complex structures of ALKBH2 mutants cross-linked to dsDNA reveal the conformational swing of beta-hairpin".SCIENCE CHINA-CHEMISTRY 57.2(2014):307-313. |
入库方式： OAI收割
来源：上海药物研究所
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