WSS25 inhibits Dicer, downregulating microRNA-210, which targets Ephrin-A3, to suppress human microvascular endothelial cell (HMEC-1) tube formation
文献类型：期刊论文
| 作者 | Xiao, Fei2; Qiu, Hong2; Zhou, Ling2; Shen, Xiaokun2; Yang, Liping1; Ding, Kan2
|
| 刊名 | GLYCOBIOLOGY
 |
| 出版日期 | 2013-05 |
| 卷号 | 23期号:5页码:524-535 |
| 关键词 | angiogenesis Dicer Ephrin-A3 miR-210 sulfated glucan |
| ISSN号 | 0959-6658 |
| DOI | 10.1093/glycob/cwt004 |
| 文献子类 | Article |
| 英文摘要 | WSS25 is a sulfated polysaccharide that inhibits angiogenesis. However, the mechanism underlying the regulation of angiogenesis by WSS25 is not well understood. Using microRNA (miRNA) microarray analysis, a total of 25 miRNAs were found to be upregulated and 12 (including miR-210) downregulated by WSS25 in human microvascular endothelial cells (HMEC-1). Interestingly, Dicer, a key enzyme for miRNA biosynthesis, was downregulated by WSS25 in HMEC-1 cells. Further studies indicated that HMEC-1 cell tube formation and miR-210 expression were suppressed while Ephrin-A3 expression was enhanced by the silencing of Dicer. In contrast, HMEC-1 cell tube formation and miR-210 expression were induced while Ephrin-A3 expression was suppressed by Dicer overexpression. Moreover, miR-210 was downregulated while Ephrin-A3 was upregulated by WSS25 in HMEC-1 cells. HMEC-1 cell migration and tube formation were arrested, while Ephrin-A3 expression was augmented by anti-miR-210. In addition, HMEC-1 cell tube formation was significantly attenuated or augmented when Ephrin-A3 was overexpressed or silenced, respectively. Nevertheless, the tube formation blocked by WSS25 could be partially rescued by manipulation of Dicer, miR-210 and Ephrin-A3. These results suggest a new pathway whereby WSS25 inhibits angiogenesis via suppression of Dicer, leading to downregulation of miR-210 and upregulation of Ephrin-A3. |
| WOS关键词 | GENE-EXPRESSION ; CANCER-CELLS ; GLUTAMATE TRANSPORT ; LIGAND EPHRIN-A3 ; LUNG-CANCER ; ANGIOGENESIS ; HYPOXIA ; GROWTH ; MIR-210 ; METASTASIS |
| 资助项目 | National Natural Science Foundation of China (NSFC)[81171914] ; National Natural Science Foundation of China (NSFC)[31230022] ; New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; National Science Fund for Distinguished Young Scholars in China[81125025] ; Industry-University-Research Institute Alliance Fund in Guangdong Province, China[2010A090200041] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000316964600003 |
| 出版者 | OXFORD UNIV PRESS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277645]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Yang, Liping |
| 作者单位 | 1.Nantong Univ, Tumor Hosp, Key Lab Canc Res Ctr Nantong, Nantong 226361, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xiao, Fei,Qiu, Hong,Zhou, Ling,et al. WSS25 inhibits Dicer, downregulating microRNA-210, which targets Ephrin-A3, to suppress human microvascular endothelial cell (HMEC-1) tube formation[J]. GLYCOBIOLOGY,2013,23(5):524-535. |
| APA | Xiao, Fei,Qiu, Hong,Zhou, Ling,Shen, Xiaokun,Yang, Liping,&Ding, Kan.(2013).WSS25 inhibits Dicer, downregulating microRNA-210, which targets Ephrin-A3, to suppress human microvascular endothelial cell (HMEC-1) tube formation.GLYCOBIOLOGY,23(5),524-535. |
| MLA | Xiao, Fei,et al."WSS25 inhibits Dicer, downregulating microRNA-210, which targets Ephrin-A3, to suppress human microvascular endothelial cell (HMEC-1) tube formation".GLYCOBIOLOGY 23.5(2013):524-535. |
入库方式： OAI收割
来源：上海药物研究所
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