Proteome-wide Quantification and Characterization of Oxidation-Sensitive Cysteines in Pathogenic Bacteria
文献类型：期刊论文
| 作者 | Deng, Xin4,7; Weerapana, Eranthie3,5,6; Ulanovskaya, Olesya5,6; Sun, Fei4,7; Liang, Haihua4,7; Ji, Quanjiang4,7; Ye, Yan2; Fu, Ye4,7; Zhou, Lu4,7; Li, Jiaxin2 |
| 刊名 | CELL HOST & MICROBE
 |
| 出版日期 | 2013-03-13 |
| 卷号 | 13期号:3页码:358-370 |
| ISSN号 | 1931-3128 |
| DOI | 10.1016/j.chom.2013.02.004 |
| 文献子类 | Article |
| 英文摘要 | Thiol-group oxidation of active and allosteric cysteines is a widespread regulatory posttranslational protein modification. Pathogenic bacteria, including Pseudomonas aeruginosa and Staphylococcus aureus, use regulatory cysteine oxidation to respond to and overcome reactive oxygen species (ROS) encountered in the host environment. To obtain a proteome-wide view of oxidation-sensitive cysteines in these two pathogens, we employed a competitive activity-based protein profiling approach to globally quantify hydrogen peroxide (H2O2) reactivity with cysteines across bacterial proteomes. We identified similar to 200 proteins containing H2O2-sensitive cysteines, including metabolic enzymes, transcription factors, and uncharacterized proteins. Additional biochemical and genetic studies identified an oxidation-responsive cysteine in the master quorum-sensing regulator LasR and redox-regulated activities for acetaldehyde dehydrogenase ExaC, arginine deiminase ArcA, and glyceraldehyde 3-phosphate dehydrogenase. Taken together, our data indicate that pathogenic bacteria exhibit a complex, multilayered response to ROS that includes the rapid adaption of metabolic pathways to oxidative-stress challenge. |
| WOS关键词 | PSEUDOMONAS-AERUGINOSA ; STAPHYLOCOCCUS-AUREUS ; HYDROGEN-PEROXIDE ; TERMINAL ALKYNES ; VIRULENCE GENES ; LARGE-SCALE ; IN-VIVO ; STRESS ; CELLS ; DEHYDROGENASE |
| 资助项目 | National Institutes of Health[AI074658] ; National Institutes of Health[P50GM081892] ; National Institutes of Health[CA087660] ; National Institutes of Health[AI101973-01] ; Burroughs Wellcome Fund Investigators in the Pathogenesis of Infectious Disease Award[00000000] ; Flight Attendant Medical Research Institute (FAMRI)[103007] |
| WOS研究方向 | Microbiology ; Parasitology ; Virology |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000330850400016 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277696]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Cravatt, Benjamin F. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58203 USA; 3.Boston Coll, Dept Chem, Chestnut Hill, MA 02467 USA; 4.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 5.Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92121 USA; 6.Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92121 USA; 7.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 8.Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA; |
| 推荐引用方式 GB/T 7714 | Deng, Xin,Weerapana, Eranthie,Ulanovskaya, Olesya,et al. Proteome-wide Quantification and Characterization of Oxidation-Sensitive Cysteines in Pathogenic Bacteria[J]. CELL HOST & MICROBE,2013,13(3):358-370. |
| APA | Deng, Xin.,Weerapana, Eranthie.,Ulanovskaya, Olesya.,Sun, Fei.,Liang, Haihua.,...&He, Chuan.(2013).Proteome-wide Quantification and Characterization of Oxidation-Sensitive Cysteines in Pathogenic Bacteria.CELL HOST & MICROBE,13(3),358-370. |
| MLA | Deng, Xin,et al."Proteome-wide Quantification and Characterization of Oxidation-Sensitive Cysteines in Pathogenic Bacteria".CELL HOST & MICROBE 13.3(2013):358-370. |
入库方式： OAI收割
来源：上海药物研究所
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