High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway
文献类型：期刊论文
| 作者 | Zhou, Yueyang2; Yu, Jing1,3; Lei, Xiling2; Wu, Jianying4; Niu, Qi2; Zhang, Yixuan4; Liu, Hong5 ; Christen, Philipp6; Gehring, Heinz6; Wu, Fang2
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| 刊名 | CHEMICAL COMMUNICATIONS
 |
| 出版日期 | 2013 |
| 卷号 | 49期号:100页码:11782-11784 |
| ISSN号 | 1359-7345 |
| DOI | 10.1039/c3cc46719h |
| 文献子类 | Article |
| 英文摘要 | We report a high-throughput assay for H2S-producing enzymes, which is based on a newly designed tandem-well plate. Screening of 21599 agents identified several potent inhibitors of cystathionine beta-synthase and cystathionine gamma-lyase, the two key enzymes generating H2S in mammals, with IC50 values in the low two-digit micromolar range. |
| WOS关键词 | CYSTATHIONINE-BETA-SYNTHASE ; GAMMA-CYSTATHIONASE ; ESCHERICHIA-COLI ; PROTEIN ; DRUGS ; LYASE ; RAT |
| 资助项目 | National Basic Research Program of China[2012CB822103] ; National Natural Science Foundation[31270853] ; National Natural Science Foundation[81102377] ; National Natural Science Foundation[31200640] ; Shanghai Pujiang Program[12PJ1405000] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000327500500020 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277777]  |
| 专题 | 药物化学研究室 |
| 通讯作者 | Wu, Fang |
| 作者单位 | 1.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China; 2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai 200240, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China; 4.Shenyang Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Shenyang 110016, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 6.Univ Zurich, Dept Biochem, CH-8057 Zurich, Switzerland |
| 推荐引用方式 GB/T 7714 | Zhou, Yueyang,Yu, Jing,Lei, Xiling,et al. High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway[J]. CHEMICAL COMMUNICATIONS,2013,49(100):11782-11784. |
| APA | Zhou, Yueyang.,Yu, Jing.,Lei, Xiling.,Wu, Jianying.,Niu, Qi.,...&Wu, Fang.(2013).High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway.CHEMICAL COMMUNICATIONS,49(100),11782-11784. |
| MLA | Zhou, Yueyang,et al."High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway".CHEMICAL COMMUNICATIONS 49.100(2013):11782-11784. |
入库方式： OAI收割
来源：上海药物研究所
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