Quantitative Acetylome Analysis Reveals the Roles of SIRT1 in Regulating Diverse Substrates and Cellular Pathways
文献类型：期刊论文
| 作者 | Chen, Yue3; Zhao, Wenhui1; Yang, Jeong Soo3; Cheng, Zhongyi3; Luo, Hao3; Lu, Zhike3; Tan, Minjia2,3 ; Gu, Wei1; Zhao, Yingming2,3
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| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
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| 出版日期 | 2012-10 |
| 卷号 | 11期号:10页码:1048-1062 |
| ISSN号 | 1535-9476 |
| DOI | 10.1074/mcp.M112.019547 |
| 文献子类 | Article |
| 英文摘要 | Despite of the progress in identifying many Lys acetylation (Kac) proteins, Kac substrates for Kac-regulatory enzymes remain largely unknown, presenting a major knowledge gap in Kac biology. Here we identified and quantified 4623 Kac sites in 1800 Kac proteins in SIRT1(+/+) and SIRT1(-/-) MEF cells, representing the first study to reveal an enzyme-regulated Kac subproteome and the largest Lys acetylome reported to date from a single study. Four hundred eighty-five Kac sites were enhanced by more than 100% after SIRT1 knockout. Our results indicate that SIRT1 regulates the Kac states of diverse cellular pathways. Interestingly, we found that a number of acetyltransferases and major acetyltransferase complexes are targeted by SIRT1. Moreover, we showed that the activities of the acetyltransferases are regulated by SIRT1-mediated deacetylation. Taken together, our results reveal the Lys acetylome in response to SIRT1, provide new insights into mechanisms of SIRT1 function, and offer biomarker candidates for the clinical evaluation of SIRT1-activator compounds. Molecular & Cellular Proteomics 11: 10.1074/mcp.M112.019547, 1048-1062, 2012. |
| WOS关键词 | HISTONE ACETYLTRANSFERASE ACTIVITY ; LYSINE ACETYLATION ; DEPENDENT TRANSCRIPTION ; DEACETYLASE INHIBITORS ; P-TEFB ; PROTEIN ; PHOSPHORYLATION ; CHROMATIN ; P53 ; COMPLEX |
| 资助项目 | National Cancer Institute of the National Institutes of Health[CA126832] ; National Cancer Institute of the National Institutes of Health[RR020839] ; National Cancer Institute of the National Institutes of Health[R01CA098821] ; National Cancer Institute of the National Institutes of Health[CA085533] ; Nancy and Leonard Florsheim Family Fund[00000000] ; NIH[T32 HL094282] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000310211000015 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277916]  |
| 专题 | 化学蛋白质组学研究中心 |
| 通讯作者 | Gu, Wei |
| 作者单位 | 1.Columbia Univ, Coll Phys & Surg, Inst Canc Genet, New York, NY 10032 USA; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA; |
| 推荐引用方式 GB/T 7714 | Chen, Yue,Zhao, Wenhui,Yang, Jeong Soo,et al. Quantitative Acetylome Analysis Reveals the Roles of SIRT1 in Regulating Diverse Substrates and Cellular Pathways[J]. MOLECULAR & CELLULAR PROTEOMICS,2012,11(10):1048-1062. |
| APA | Chen, Yue.,Zhao, Wenhui.,Yang, Jeong Soo.,Cheng, Zhongyi.,Luo, Hao.,...&Zhao, Yingming.(2012).Quantitative Acetylome Analysis Reveals the Roles of SIRT1 in Regulating Diverse Substrates and Cellular Pathways.MOLECULAR & CELLULAR PROTEOMICS,11(10),1048-1062. |
| MLA | Chen, Yue,et al."Quantitative Acetylome Analysis Reveals the Roles of SIRT1 in Regulating Diverse Substrates and Cellular Pathways".MOLECULAR & CELLULAR PROTEOMICS 11.10(2012):1048-1062. |
入库方式： OAI收割
来源：上海药物研究所
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