Detecting metastasis of gastric carcinoma using high-resolution micro-CT system: In vivo small animal study
文献类型：会议论文
| 作者 | Liu, Junting5; Tian, Jie4,5; Liang, Jimin5; Li, Xiangsi5; Yang, Xiang1; Chen, Xiaofeng1; Chen, Yi2 ; Zhou, Yuanfeng2; Wang, Xiaorui3
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| 出版日期 | 2011 |
| 卷号 | 7965 |
| DOI | 10.1117/12.878410 |
| 英文摘要 | Immunocytochemical and immunofluorescence staining are used for identifying the characteristics of metastasis in traditional ways. Micro-computed tomography (micro-CT) is a useful tool for monitoring and longitudinal imaging of tumor in small animal in vivo. In present study, we evaluated the feasibility of the detection for metastasis of gastric carcinoma by high-resolution micro-CT system with omnipaque accumulative enhancement method in the organs. Firstly, a high-resolution micro-CT ZKKS-MCT-sharp micro-CT was developed by our research group and Guangzhou Zhongke Kaisheng Medical Technology Co., Ltd. Secondly, several gastric carcinoma models were established through inoculating 2×106BGC-823 gastric carcinoma cells subcutaneously. Thirdly, micro-CT scanning was performed after accumulative enhancement method of intraperitoneal injection of omnipaque contrast agent containing 360 mg iodine with a concentration of 350 mg I/ml. Finally, we obtained high-resolution anatomical information of the metastasis in vivo in a BALB/c NuNu nude mouse, the 3D tumor architecture is revealed in exquisite detail at about 35 μm spatial resolution. In addition, the accurate shape and volume of the micrometastasis as small as 0.78 mm3can be calculated with our software. Overall, our data suggest that this imaging approach and system could be used to enhance the understanding of tumor proliferation, metastasis and could be the basis for evaluating anti-tumor therapies. |
| 会议录 | Progress in Biomedical Optics and Imaging - Proceedings of SPIE
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| 会议录出版者 | SPIE |
| 文献子类 | Proceedings Paper |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266856]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Tian, Jie |
| 作者单位 | 1.Guangzhou Zhongke Kaisheng Medical Technology Co., Ltd., Guangzhou 510663, China; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 3.School of Technical Physics, Xidian University, Xi'an 710071, China 4.Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; 5.School of Life Sciences and Technology, Xidian University, Xi'an 710071, China; |
| 推荐引用方式 GB/T 7714 | Liu, Junting,Tian, Jie,Liang, Jimin,et al. Detecting metastasis of gastric carcinoma using high-resolution micro-CT system: In vivo small animal study[C]. 见:. |
入库方式： OAI收割
来源：上海药物研究所
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