Determination of salbutamol in human plasma after aerosol inhalation using hydrophilic interaction chromatography (HILIC) - tandem mass spectrometry
文献类型：期刊论文
| 作者 | Ma Minkang1; Chen Xiaoyan2 ; Guo Lixia2; Zhang Yifan2; Zhong Dafang1
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2013 |
| 卷号 | 33期号:6页码:929-934 |
| 关键词 | salbutamol aerosol hydrophilic interaction chromatography!tandem mass spectrometry plasma concentration clinical pharmacokinetics |
| ISSN号 | 0254-1793 |
| 其他题名 | 亲水作用色谱-串联质谱法测定气雾剂给药后人血浆中的沙丁胺醇 |
| 文献子类 | Article |
| 英文摘要 | Objective: To develop and validate a sensitive and rapid hydrophilic interaction chromatography!tandem mass spectrometric (HILIC!MS/MS)method for the determination of salbutamol in human plasma after aerosol inhalation. Methods: Samples were prepared from plasma by protein precipitation using acetonitrile as the precipitation solvent; salbutamol and d9!salbutamol were separated on a Luna HILIC column (100 mm*3.0 mm, 3 mum)using acetonitrile (consisting of 0.1% formic acid)- 5 mmol·L~(-1)ammonium acetate (pH 8. 5 adjusted with ammonia water)(93: 7, v/v)as a mobile phase with isocratic elution. The injection volume was 2.0 muL, and a chromatographic total run time was 3.5 min. Mass spectrometry detection was conducted through electrospray ionization in positive ion multiple reaction monitoring modes, using the transitions of m/z 240 (148 + 166)and m/z 249 (149 + 167)for salbutamol and d_9-salbutamol, respectively. Results: The method was linear over the concentration range of 11.7-2340 pg·mL~(-1)and the lower limit of quantification was 11.7 pg·mL~(-1). The intra-and inter-day precision in terms of the relative standard deviation were less than 14.1%. The accuracy was in a range of ! 3.8% to !0.8% in terms of relative error. The method was successfully applied to pharmacokinetic evaluation study in healthy volunteers following inhalation of 200 mug salbutamol aerosol. Conclusion: The established HILIC-MS/ MS method is sensitive, rapid and accuracy, which is suitable for the determination of salbutamol in human plasma and can detect the plasma concentration of 24 h post-dose. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4871313 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267775]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; 2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Ma Minkang,Chen Xiaoyan,Guo Lixia,et al. Determination of salbutamol in human plasma after aerosol inhalation using hydrophilic interaction chromatography (HILIC) - tandem mass spectrometry[J]. Chinese Journal of Pharmaceutical Analysis,2013,33(6):929-934. |
| APA | Ma Minkang,Chen Xiaoyan,Guo Lixia,Zhang Yifan,&Zhong Dafang.(2013).Determination of salbutamol in human plasma after aerosol inhalation using hydrophilic interaction chromatography (HILIC) - tandem mass spectrometry.Chinese Journal of Pharmaceutical Analysis,33(6),929-934. |
| MLA | Ma Minkang,et al."Determination of salbutamol in human plasma after aerosol inhalation using hydrophilic interaction chromatography (HILIC) - tandem mass spectrometry".Chinese Journal of Pharmaceutical Analysis 33.6(2013):929-934. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。