Liquid chromatography-tandem mass spectrometry simultaneous determination of repaglinide and metformin in human plasma and its application to bioequivalence study
文献类型：期刊论文
| 作者 | Liang XR(梁晓荣); Dai XJ(戴晓健) ; Zhang YF(张逸凡); Ding JF(丁珏芳); Chen XY(陈笑艳); Zhong DF(钟大放)
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| 刊名 | Acta Pharmaceutica Sinica
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| 出版日期 | 2013 |
| 卷号 | 48期号:4页码:547-553 |
| 关键词 | repaglinide metformin LC-MS/MS LC-MS/MS |
| ISSN号 | 0513-4870 |
| 其他题名 | LC-MS/MS法同时测定人血浆中瑞格列奈和二甲双胍及其在人体生物等效性研究中的应用 |
| 文献子类 | Article |
| 英文摘要 | A simple, sensitive, selective, and reproducible liquid chromatography-tandem mass spectrometric method was developed for the simultaneous determination of repaglinide and metformin in human plasma using d_5-repaglinide and d_6-metformin as internal standards (ISs). After a simple protein precipitation using acetonitrile as the precipitation solvent, both analytes and ISs were separated on a Venusil ASB C_(18) (150 mm * 4.6 mm, 5 mum) via gradient elution using acetonitrile ? 10 mmol·L~(?1) ammonium acetate as the mobile phase. A chromatographic total run time of 7.5 min was achieved. Mass spectrometric detection was conducted with atmospheric pressure chemical ionization under positive-ion and multiple-reaction monitoring modes. The method was linear over the 0.2 to 60.0 ng·mL~(?1) concentration range for repaglinide and over the 4 to 1 000 ng·mL~(?1) range for metformin. For both analytes, the intra- and inter-accuracies and precisions were within the 15% acceptable limit across all concentrations. The validated method was successfully applied to a clinical bioequivalence study. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4815811 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267786]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong DF(钟大放) |
| 作者单位 | 1.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; 2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Liang XR,Dai XJ,Zhang YF,et al. Liquid chromatography-tandem mass spectrometry simultaneous determination of repaglinide and metformin in human plasma and its application to bioequivalence study[J]. Acta Pharmaceutica Sinica,2013,48(4):547-553. |
| APA | 梁晓荣,戴晓健,张逸凡,丁珏芳,陈笑艳,&钟大放.(2013).Liquid chromatography-tandem mass spectrometry simultaneous determination of repaglinide and metformin in human plasma and its application to bioequivalence study.Acta Pharmaceutica Sinica,48(4),547-553. |
| MLA | 梁晓荣,et al."Liquid chromatography-tandem mass spectrometry simultaneous determination of repaglinide and metformin in human plasma and its application to bioequivalence study".Acta Pharmaceutica Sinica 48.4(2013):547-553. |
入库方式： OAI收割
来源：上海药物研究所
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