Simultaneous determination of erdosteine and its active metabolite in human plasma by liquid chromatography-tandem mass spectrometry with pre-column derivatization
文献类型：期刊论文
| 作者 | Jin Jing2; Chen Xiaoyan1 ; Zhang Yifan1; Ma Zhiyu1; Zhong Dafang2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2013 |
| 卷号 | 48期号:3页码:395-400 |
| 关键词 | LC-MS/MS erdosteine pharmacokinetics active metabolite |
| ISSN号 | 0513-4870 |
| 其他题名 | 柱前衍生化LC-MS/MS法同时测定人血浆中厄多司坦及其活性代谢物 |
| 文献子类 | Article |
| 英文摘要 | A sensitive, rapid and accurate liquid chromatography-tandem mass spectrometric (LC-MS/MS) method with pre-column derivatization was developed for the simultaneous determination of erdosteine and its thiol-containing active metabolite in human plasma. Paracetamol and captopril were chosen as the internal standard of erdosteine and its active metabolite, respectively. Aliquots of 100 muL plasma sample were derivatized by 2-bromine-3'-methoxy acetophenone, then separated on an Agilent XDB-C_(18) (50 mm * 4.6 mm ID, 1.8 mum) column using 0.1% formic acid methanol ? 0.1% formic acid 5 mmol·L~(-1) ammonium acetate as mobile phase, in a gradient mode. Detection of erdosteine and its active metabolite were achieved by ESI MS/MS in the positive ion mode. The linear calibration curves for erdosteine and its active metabolite were obtained in the concentration ranges of 5 ? 3 000 ng·mL~(-1) and 5 ? 10 000 ng·mL~(-1), respectively. The lower limit of quantification of erdosteine and its active metabolite were both 5.00 ng·mL~(-1). The pharmacokinetic results of erdosteine and its thiol-containing active metabolite showed that the area under curve (AUC) of the thiol-containing active metabolite was 6.2 times of that of erdosteine after a single oral dose of 600 mg erdosteine tables in 32 healthy volunteers, The mean residence time (MRT) of the thiol-containing active metabolite was (7.510.788) h, which provided a pharmacokinetic basis for the rational dosage regimen. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4775217 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267792]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Soochow University, Suzhou, Jiangsu 215123, China.; |
| 推荐引用方式 GB/T 7714 | Jin Jing,Chen Xiaoyan,Zhang Yifan,et al. Simultaneous determination of erdosteine and its active metabolite in human plasma by liquid chromatography-tandem mass spectrometry with pre-column derivatization[J]. Acta Pharmaceutica Sinica,2013,48(3):395-400. |
| APA | Jin Jing,Chen Xiaoyan,Zhang Yifan,Ma Zhiyu,&Zhong Dafang.(2013).Simultaneous determination of erdosteine and its active metabolite in human plasma by liquid chromatography-tandem mass spectrometry with pre-column derivatization.Acta Pharmaceutica Sinica,48(3),395-400. |
| MLA | Jin Jing,et al."Simultaneous determination of erdosteine and its active metabolite in human plasma by liquid chromatography-tandem mass spectrometry with pre-column derivatization".Acta Pharmaceutica Sinica 48.3(2013):395-400. |
入库方式： OAI收割
来源：上海药物研究所
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