The effect of the location of purification tag fusion on chromatography behavior and activity of recombinant scorpion venom peptides
文献类型：期刊论文
| 作者 | Hui Changye3; Shao Jianhua1; Guo Yan4; Zhang Xi2; Yang Xueqin3; Wang Dianpeng3 |
| 刊名 | Chinese Medicinal Biotechnolog
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:6页码:436-440 |
| 关键词 | Charydotoxin Recombinant fusion proteins Molecular conformation Chromatography behavior iological activity |
| ISSN号 | 1673-713X |
| 其他题名 | 纯化标签不同融合端的选择对蝎毒肽色谱行为的影响 |
| 文献子类 | Article |
| 英文摘要 | Objective To investigate the chromatography behavior of N- or C-terminal His tag fusion recombinant anti-epilepsy peptide (AEP). Methods Three dimensional model of AEP was built by homology-based method. The shielding effect of His tag by spatial conformation was predicted, and fusion peptides were successfully expressed and purified. Biological activity of them were assayed. Results A conformational analysis of AEP showed that the space shielding effect of the C-terminal region of fusion tag was less potent than that of the N-terminus. Under the premise of the same expression amount and chromatographic conditions of N- or C-terminal His tag fusion recombinant AEP, the production of AEP-His6 and Met-Gly-His6-AEP was 3.3 mg and 0.8 mg per liter fermentation broth, respectively. The effect on rAEP activity by the His tag C-terminal fusions was less significant than that of the N-terminal fusion. Conclusions The place of His tag at N- or C-terminus has significant impact on chromatographic behavior and activity of small molecule active peptides like scorpion venom which possesses simple spatial conformation. |
| 资助项目 | 中国第43批博士后基金资助[00000000] ; 教育部重点实验室项目[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4706083 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267808]  |
| 专题 | 药理学第一研究室 |
| 作者单位 | 1.College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, Jiangsu 225009, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 3.Division of Toxicology and Pathology, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen 518001.; 4.Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518054, China.; |
| 推荐引用方式 GB/T 7714 | Hui Changye,Shao Jianhua,Guo Yan,et al. The effect of the location of purification tag fusion on chromatography behavior and activity of recombinant scorpion venom peptides[J]. Chinese Medicinal Biotechnolog,2012,7(6):436-440. |
| APA | Hui Changye,Shao Jianhua,Guo Yan,Zhang Xi,Yang Xueqin,&Wang Dianpeng.(2012).The effect of the location of purification tag fusion on chromatography behavior and activity of recombinant scorpion venom peptides.Chinese Medicinal Biotechnolog,7(6),436-440. |
| MLA | Hui Changye,et al."The effect of the location of purification tag fusion on chromatography behavior and activity of recombinant scorpion venom peptides".Chinese Medicinal Biotechnolog 7.6(2012):436-440. |
入库方式： OAI收割
来源：上海药物研究所
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