Biotransformation of triptolide to (5R)-5-hydroxytriptolide
文献类型：期刊论文
| 作者 | Zhao Shunbo2; Huang Haihua1; Chen Xiaoyan1 ; Li Xiuli1; Zhong Dafang2
|
| 刊名 | Chinese Journal New Drugs
 |
| 出版日期 | 2012 |
| 卷号 | 21期号:3页码:303-309 |
| 关键词 | (5R)-5-hydroxytriptolide triptolide microbial transformation Cunninghamella elegans HPLC |
| ISSN号 | 1003-3734 |
| 其他题名 | 微生物转化法从雷公藤甲素合成雷腾舒 |
| 文献子类 | Article |
| 英文摘要 | Objective: To synthesize(5R)-5-hydroxytriptolide by microbial transformation of triptolide.Methods: Among 10 strains of Cunninghamella screened,Cunninghamella elegans ATCC36112 was selected as a suitable bioconverter.To calculate the transformation yield,the concentration of(5R)-5-hydroxytriptolide in the fermentation fluids was determined by HPLC.Factors influencing biotransformation system were optimized to increase the yield of(5R)-5-hydroxytriptolide.Results: The optimized conditions were pH 4.5,400 mug·mL~(-1) of substrate concentration and 10 d of transformation.A yield of > 70% was achieved.Conclusion: We have proved that biotransformation of triptolide to(5R)-5-hydroxytriptolide by Cunninghamella elegans ATCC36112 is a new approach for the synthesis of(5R)-5-hydroxytriptolide. |
| 资助项目 | 国家自然科学基金[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4453907 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267848]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; |
| 推荐引用方式 GB/T 7714 | Zhao Shunbo,Huang Haihua,Chen Xiaoyan,et al. Biotransformation of triptolide to (5R)-5-hydroxytriptolide[J]. Chinese Journal New Drugs,2012,21(3):303-309. |
| APA | Zhao Shunbo,Huang Haihua,Chen Xiaoyan,Li Xiuli,&Zhong Dafang.(2012).Biotransformation of triptolide to (5R)-5-hydroxytriptolide.Chinese Journal New Drugs,21(3),303-309. |
| MLA | Zhao Shunbo,et al."Biotransformation of triptolide to (5R)-5-hydroxytriptolide".Chinese Journal New Drugs 21.3(2012):303-309. |
入库方式： OAI收割
来源：上海药物研究所
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