Enantioselective determinination of R-warfarin/S-warfarin in human plasma using liquid chromatography-tandem mass spectrometry and its application in a drug-drug interaction study
文献类型：期刊论文
| 作者 | Jin Shu2; Zhang Yifan1 ; Chen Xiaoyan1; Liu Ke1; Zhong Dafang2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2012 |
| 卷号 | 47期号:1页码:105-109 |
| 关键词 | LC-MS/MS LC-MS/MS warfarin morinidazole drug-drug interaction |
| ISSN号 | 0513-4870 |
| 其他题名 | 对映体选择性LC-MS/MS法测定人血浆中R/S-华法林及其在药物相互作用研究中的应用 |
| 文献子类 | Article |
| 英文摘要 | To study the drug-drug interaction of morinidazole and warfarin and its application,a sensitive and rapid liquid chromatography-tandem mass spectrometric(LC-MS/MS) method was developed for the determination of R-warfarin/S-warfarin in human plasma.In a random,two-period crossover study,12 healthy volunteers received a single oral dose of 5 mg racemic warfarin in the absence and presence of morinidazole.Blood samples were collected according to a pre-designed time schedule.R-warfarin,S-warfarin and methyclothiazide were extracted with ethylether∶methylenechloride(3∶2),then separated on a Astec Chirobiotic~(TM) V(150 mm * 4.6 mm ID,5 mum) column using 5 mmol·L~(-1) ammonium acetate(pH 4.0) - acetonitrile as mobile phase at a flow-rate of 1.5 mL-min~(-1).The mobile phase was splitted and 0.5 mL·min~(-1) was introduced into MS.A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the negative ion mode.Quantification was performed using multiple reaction monitoring(MRM).The resolution of warfarin enantiomers is 1.56.The linear calibration curves for R-warfarin and S-warfarin both were obtained in the concentration range of 5 - 1 000 ng·mL~(-1).Intra-and inter-day relative standard deviation(RSD) for R-warfarin and S-warfarin over the entire concentration range across three validation runs was both less than 10%,and relative error(RE) ranged from -4.9% to 0.7%,separately.The method herein described is effective and convenient,and suitable for the study of metabolic interaction between morinidazole and warfarin.The results showed that coadministration of warfarin with morinidazole did not affect the pharmacokinetics of either R-warfarin or S-warfarin. |
| 资助项目 | 国家科技重大专项资助项目[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4438152 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267853]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. 2.Soochow University, Suzhou, Jiangsu 215123, China.; |
| 推荐引用方式 GB/T 7714 | Jin Shu,Zhang Yifan,Chen Xiaoyan,et al. Enantioselective determinination of R-warfarin/S-warfarin in human plasma using liquid chromatography-tandem mass spectrometry and its application in a drug-drug interaction study[J]. Acta Pharmaceutica Sinica,2012,47(1):105-109. |
| APA | Jin Shu,Zhang Yifan,Chen Xiaoyan,Liu Ke,&Zhong Dafang.(2012).Enantioselective determinination of R-warfarin/S-warfarin in human plasma using liquid chromatography-tandem mass spectrometry and its application in a drug-drug interaction study.Acta Pharmaceutica Sinica,47(1),105-109. |
| MLA | Jin Shu,et al."Enantioselective determinination of R-warfarin/S-warfarin in human plasma using liquid chromatography-tandem mass spectrometry and its application in a drug-drug interaction study".Acta Pharmaceutica Sinica 47.1(2012):105-109. |
入库方式： OAI收割
来源：上海药物研究所
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