Simultaneous determination of ceftazidime and tazobactam in human plasma with sequential positive and negative ionization liquid chromatography-tandem mass spectrometric detection
文献类型：期刊论文
| 作者 | Zhang Juan1 ; Chen Xiaoyan3; Dai Xiaojian3; Zhang Yifan3; Liu Ke3; Li Zhen4; Lin Changzheng2; Zhong Dafang1
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| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2012 |
| 卷号 | 32期号:1页码:20 |
| 关键词 | liquid chromatographic-tandem mass spectrometry biological sample ceftazidime tazobactam internal standard cefadroxil sulbactam antibiotics pharmacokinetics |
| ISSN号 | 0254-1793 |
| 其他题名 | 正负离子切换液相色谱-串联质谱法同时测定人血浆中头孢他啶和他唑巴坦 |
| 文献子类 | Article |
| 英文摘要 | Objective:To develop a sensitive and specific liquid chromatographic-tandem mass spectrometric(LC-MS/MS) method for simultaneous determination of ceftazidime and tazobactam in human plasma and applied to the pharmacokinetic study.Methods:The plasma sample was extracted with acetonitrile,then separated on a Venusil ASB-C_(18) column(150 mm*4.6 mm,5 mum) using acetonitrile-5 mmol·L-1 ammonium acetate-formic acid(20∶ 80∶ 0.16,v/v/v) as mobile phase.Ceftazidime was ionized under positive ESI condition,while tazobactam required negative ESI condition in order to improve sensitivity.The witch time from positive ion to negative ion was at 3.8 min.The retention times for ceftazidime and cefadroxil(internal standard) were 3.0 min and 2.8 min,respectively.The retention times for tazobactam and sulbactam(internal standard) were 4.4 min and 4.9 min,respectively.Multiple reaction monitoring(MRM) using the precursor to product ion of m/z 547468,m/z 299138,m/z 364208 and m/z 232140 were performed to quantify ceftazidime,tazobactam,internal standard cefadroxil and sulbactam,respectively.Results:The linear calibration curves for ceftazidime and tazobactam were obtained in the concentration range of 0.250-250 mug·mL~(-1) and 0.0250-25.0 mug·mL~(-1).Intra-and inter-day relative standard deviation(RSD) for ceftazidime and tazobactam over the entire concentration across three validation runs was both less than 10.8%,and relative error(RE) ranged from-7.6% to 2.1%.The method was successfully applied to a pharmacokinetic study of intravenous drip of three doses of ceftazidime/tazobactam sodium(containing ceftazidime/tazobactam at 1 g/0.167 g,2 g/0.333 g,4 g/0.667 g) in healthy volunteers.Conclusion:The method herein described is effective,convenient and suitable for the clinical pharmacokinetic study of ceftazidime/tazobactam. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4432700 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267855]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Soochow University, Suzhou, Jiangsu 215000, China.; 2.Xiangbei Welman Pharmaceutical Co.,Ltd, Changsha, Hunan 410330, China. 3.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China.; 4.Changhai Hospital,Second Military Medical University, Shanghai 200433, China.; |
| 推荐引用方式 GB/T 7714 | Zhang Juan,Chen Xiaoyan,Dai Xiaojian,et al. Simultaneous determination of ceftazidime and tazobactam in human plasma with sequential positive and negative ionization liquid chromatography-tandem mass spectrometric detection[J]. Chinese Journal of Pharmaceutical Analysis,2012,32(1):20. |
| APA | Zhang Juan.,Chen Xiaoyan.,Dai Xiaojian.,Zhang Yifan.,Liu Ke.,...&Zhong Dafang.(2012).Simultaneous determination of ceftazidime and tazobactam in human plasma with sequential positive and negative ionization liquid chromatography-tandem mass spectrometric detection.Chinese Journal of Pharmaceutical Analysis,32(1),20. |
| MLA | Zhang Juan,et al."Simultaneous determination of ceftazidime and tazobactam in human plasma with sequential positive and negative ionization liquid chromatography-tandem mass spectrometric detection".Chinese Journal of Pharmaceutical Analysis 32.1(2012):20. |
入库方式： OAI收割
来源：上海药物研究所
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