LC-MS/MS determination of pramipexole in human plasma
文献类型：期刊论文
| 作者 | Li Hua3; Chen Xiaoyan1 ; Shi Xiaojin2; Zhan Yan1; Zhong Mingkang2; Li Zhongdong2; Zhong Dafang3
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2011 |
| 卷号 | 31期号:2页码:330-335 |
| 关键词 | pramipexole liquid chromatographic-tandem mass spectrometry plasma concentration |
| ISSN号 | 0254-1793 |
| 其他题名 | 高灵敏度液相色谱-串联质谱法测定人血浆中普拉克索 |
| 文献子类 | Article |
| 英文摘要 | Objective:To develop and validate a sensitive and convenient liquid chromatographic-tandem mass spectrometric(LC-MS/MS) method for the determination of pramipexole in human plasma and to study the pharmacokinetics of pramipexole. Methods:The plasma sample was extracted with ethyl ether dichlormethane(3:2,v/v), then separated on a Venusil ASB-C18(150 mm*4.6 mm,5 mum)column using acetonitrile-10 mmol·L-1 ammonium acetate-formic acid(60:40:0.1,v/v/v) as the mobile phase. A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated in the positive ion mode. Quantitation was performed using selective reaction monitoring(SRM) of the transitions of m/z 212m/z(111 + 126 +153)and m/z 243m/z 210 for pramipexole and the internal standard huperzine A, respectively. Results:The linear calibration curves were obtained in the concentration range of 5.92-740 pg·mL-1.The lower limit of quantification was 5.92 pg·mL-1.The intra-day and inter-day relative standard deviation over the entire concentration range was less than 9.7%.The accuracy was in the range of -2.5% to 0.6% in terms of relative error. The method was successfully applied to a pharmacokinetic study of pramipexole dihydrochloride monohydrate tablets containing 0.125 mg or 0.25 mg in healthy volunteers. Conclusion:Three SRM transitions are selected in the quantification of pramipexole, which improve the sensitivity of the method significantly, and the method is proved to be suitable for the determination of pramipexole in human plasma |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4126566 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267896]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Huashan Affiliated Hospital of Fudan University, Shanghai 200040, China. 3.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; |
| 推荐引用方式 GB/T 7714 | Li Hua,Chen Xiaoyan,Shi Xiaojin,et al. LC-MS/MS determination of pramipexole in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2011,31(2):330-335. |
| APA | Li Hua.,Chen Xiaoyan.,Shi Xiaojin.,Zhan Yan.,Zhong Mingkang.,...&Zhong Dafang.(2011).LC-MS/MS determination of pramipexole in human plasma.Chinese Journal of Pharmaceutical Analysis,31(2),330-335. |
| MLA | Li Hua,et al."LC-MS/MS determination of pramipexole in human plasma".Chinese Journal of Pharmaceutical Analysis 31.2(2011):330-335. |
入库方式： OAI收割
来源：上海药物研究所
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