LC-MS/MS determination of tizanidine in human plasma
文献类型：期刊论文
| 作者 | WAN Mimi1; ZHAN Yan2 ; CHEN Xiaoyan2; ZHANG Yifan2; ZHONG Dafang1
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2011 |
| 卷号 | 31期号:8页码:1435-1439 |
| 关键词 | tizanidine imidazoline derivative liquid chromatography mass spectrometry pharmacokinetics |
| ISSN号 | 0254-1793 |
| 其他题名 | 液相色谱-串联质谱法测定人血浆中替扎尼定 |
| 文献子类 | Article |
| 英文摘要 | Objective:To develop and validate a liquid chromatography-tandem mass spectrometric(LC-MS/ MS)method for the determination of tizanidine in human plasma.Methods:Tizanidine and the internal standard huperzine A were separated from plasma by alkalinized liquid-liquid extraction with diethyl ether.Chromatographic separation was performed on a Zorbax SB C18(150 mm*4.6 mm,5mum)column with an isocratic mobile phase consisting of methanoL~(-1)0 mmol·L~(-1)ammonium acetate-formic acid(55∶45∶0.1,v/v/v).A tandem mass spectrometer equipped with electrospray ionization source was adopted as detector.Selected reaction monitoring(SRM)transitions of m/z 25444 and m/z 243210 were measured in positive mode for tizanidine and internal standard,respectively.Results:The linear concentration range of the calibration curve for tizanidine was 10.0-5000 pg·mL~(-1).The lower limit of quantification was 10.0 pg·mL~(-1).The intra-day and inter-day relative standard deviation over the entire concentration range was less than 10.0%.The accuracy was within 3.6%in terms of relative error.Conclusion:The method is sensitive and suitable for pharmacokinetic study of tizanidine in human subjects. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4312614 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267910]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | ZHONG Dafang |
| 作者单位 | 1.School of Pharmacy,Soochow University, Suzhou, Jiangsu 215006, China.; 2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | WAN Mimi,ZHAN Yan,CHEN Xiaoyan,et al. LC-MS/MS determination of tizanidine in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2011,31(8):1435-1439. |
| APA | WAN Mimi,ZHAN Yan,CHEN Xiaoyan,ZHANG Yifan,&ZHONG Dafang.(2011).LC-MS/MS determination of tizanidine in human plasma.Chinese Journal of Pharmaceutical Analysis,31(8),1435-1439. |
| MLA | WAN Mimi,et al."LC-MS/MS determination of tizanidine in human plasma".Chinese Journal of Pharmaceutical Analysis 31.8(2011):1435-1439. |
入库方式： OAI收割
来源：上海药物研究所
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