The method for determination of fosfomycin in human plasma by LC-MS/MS and its application
文献类型：期刊论文
| 作者 | Liu Xinxia2; Li Li1; Yuan Shiying2; Dai Xiaojian1 ; Cao Cuiling2; Chen Xiaoyan1; Zhong Dafang1; Chen Hui2
|
| 刊名 | Chinese Journal of Hospital Pharmacy
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:18页码:1567-1570 |
| 关键词 | fosfomycin calcium human plasma HPLC-MSlMS |
| ISSN号 | 1001-5213 |
| 其他题名 | 液-质联用法测定人血浆中磷霉素的浓度及其应用 |
| 文献子类 | Article |
| 英文摘要 | OBJECTIVE To develop a liquid chromatographic/tandem mass spectrometric method for determination of fosfomycin in human plasma. METHODS 50muL fudosteine (IS solution, 1. 00 mg·L~(-1)) and 50mul plasma were mixed and deproteinized with 250 muL methanol, then 30 muL of the supernatant were further mixed with 100 muL water, and a 20 muL aliquot of mixture was injected for the LC/MS/MS analysis. Chromatographic separations were carried out on a 4. 6 mm X 100 mm Ultramatf TM XB-CN column, The isocratic mobile phase, methanol 5 mmol·L~(-1) ammonium acetate (10:90) was run at a flow rate of 0. 7 mL·min~(-1) at room temperature. An API 4000 tandem mass spectrometer as detector was operated in the negative ion mode. Multiple reaction monitoring using the precursor to product ion combinations of m/x 13779 and m/z 17891 was performed to quantify fosfomycin and fudosteine, respectively. RESULTS The linear range of concentration was 0. 10 - 12. 0 mg' L~(-1). The lower limit of quantification was 0. 100 mg·L~(-1).The intra-day and inter-day relative standard deviation was less than 10. 6%. The absolute recoveries of low, medium and high concentration were 95. 4%,95. 9%,91. 6%. CONCLUSION This method is simple, sensitive, accurate and reproducible. It is suitable for pharmacokinetic study of fosfomycin calcium |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4024822 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267922]  |
| 专题 | 上海药物代谢研究中心 |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Clinical pharmacology Institute, Tongji Medical College, HUST, Wuhan, Hubei 430030, China.; |
| 推荐引用方式 GB/T 7714 | Liu Xinxia,Li Li,Yuan Shiying,et al. The method for determination of fosfomycin in human plasma by LC-MS/MS and its application[J]. Chinese Journal of Hospital Pharmacy,2010,30(18):1567-1570. |
| APA | Liu Xinxia.,Li Li.,Yuan Shiying.,Dai Xiaojian.,Cao Cuiling.,...&Chen Hui.(2010).The method for determination of fosfomycin in human plasma by LC-MS/MS and its application.Chinese Journal of Hospital Pharmacy,30(18),1567-1570. |
| MLA | Liu Xinxia,et al."The method for determination of fosfomycin in human plasma by LC-MS/MS and its application".Chinese Journal of Hospital Pharmacy 30.18(2010):1567-1570. |
入库方式： OAI收割
来源：上海药物研究所
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