Determination of Erythromycin Cyclic 11,12-Carbonate in Human Plasma by LC-MS/MS Method
文献类型：期刊论文
| 作者 | Lin Feifei2 ; Chen Xiaoyan1; Dai Xiaojian1; Zhong Dafang2
|
| 刊名 | Chinese Pharmaceutical Journal
 |
| 出版日期 | 2010 |
| 卷号 | 45期号:13页码:1020-1023 |
| 关键词 | erythromycin cyclic 11,12-carbonate liquid chromatography-tandem mass spectrometry bioequivalence plasma concentration |
| ISSN号 | 1001-2494 |
| 其他题名 | 液相色谱-串联质谱法测定人血浆中环酯红霉素 |
| 文献子类 | Article |
| 英文摘要 | OBJECTIVE To develop a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of erythromycin cyclic 11,12-carbonate in human plasma. METHODS An aliquot of 50 muL plasma sample was extracted by n-hexane-dichloromethane-isopropanol(300: 150:15) , then separated on a Capcell Pak C_(18) MG(2. 0 mm * 35 mm, 3 mum)column using methanol-5 mmol · L~(-1) ammonium acetate-formic acid (75: 25: 0. 2). A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 761m/z 158 and m/z 838/z 158 for erythromycin cyclic 11,12-carbonate and internal standard roxithromycin as internal standard, respectively. RESULTS The linear calibration curves were obtained in the concentration range from 1. 00 to 2 000 ng · mL~(-1). The low limit of quantification was 1. 00 ng · mL~(-1) and the sensitivity was elevated by at least 20 times compared with the methods mentioned in the literature. The intra-day and inter-day relative standard deviation over the entire concentration range was below 9. 6%. The accuracy was within 0. 9% in terms of relative error. The mean recovery was 80. 4% and 79. 3% for erythromycin cyclic 11,12-carbonate and internal standard roxithromycin, respectively. The total chromatographic run time was 2. 5 min. CONCLUSION This method was sensitive, selective and rapid, and suitable to evaluate the bioequivalence of two erythromycin cyclic 11,12-carbonate tablets. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3898631 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267932]  |
| 专题 | 上海药物代谢研究中心 |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Soochow University, Suzhou, Jiangsu 215006, China.; |
| 推荐引用方式 GB/T 7714 | Lin Feifei,Chen Xiaoyan,Dai Xiaojian,et al. Determination of Erythromycin Cyclic 11,12-Carbonate in Human Plasma by LC-MS/MS Method[J]. Chinese Pharmaceutical Journal,2010,45(13):1020-1023. |
| APA | Lin Feifei,Chen Xiaoyan,Dai Xiaojian,&Zhong Dafang.(2010).Determination of Erythromycin Cyclic 11,12-Carbonate in Human Plasma by LC-MS/MS Method.Chinese Pharmaceutical Journal,45(13),1020-1023. |
| MLA | Lin Feifei,et al."Determination of Erythromycin Cyclic 11,12-Carbonate in Human Plasma by LC-MS/MS Method".Chinese Pharmaceutical Journal 45.13(2010):1020-1023. |
入库方式： OAI收割
来源：上海药物研究所
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