LC-MS/MS determination of coenzyme Q10 in human plasma
文献类型：期刊论文
| 作者 | Wang Yanping2; Dai Xiaojian1 ; Li Liang1; Chen Xiaoyan1; Zhong Dafang2
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2010 |
| 卷号 | 30期号:12页码:2251-2257 |
| 关键词 | coenzyme Q10 liquid chromatography tandem mass spectrometry plasma drug concentration pharmacokinetics |
| ISSN号 | 0254-1793 |
| 其他题名 | LC-MS/MS法测定人血浆中的辅酶Q10 |
| 文献子类 | Article |
| 英文摘要 | Objective: To develop a rapid, sensitive and convenient liquid chromatographic-tandem mass spectrometric(LC-MS/MS)method for quantification of coenzyme Q10(CoQ10)in human plasma. Methods: The plasma sample was extracted twice with n-hexane, then separated on a Capcell Pak C18(35 mm*2.0 mm,5 mum)column using methanol as the mobile phase. A triple quadrupole mass spectrometry equipped with atmospheric pressure chemical ionization(APCI)source was used as the detector and operated in the positive ion mode. Quantitation was performed using multiple reaction monitoring(MRM)of the transitions of m/z 864197 and m/z 796197 for CoQ10 and the internal standard CoQ9, respectively. Results: The linear concentration range of the calibration curve for CoQ10 was 10.01000 ng·mL-1.The lower limit of quantification was 10.0 ng·mL-1.The intra-day and inter-day relative standard deviation over the entire concentration range were less than 8.9%.The accuracy was in the range of -0.9% to 3.8% in terms of relative error. Each sample was chromatographed within 4.5 min. Conclusion: The method is sensitive, rapid and suitable for the determination of CoQ10 in human plasma and investigation of coenzyme Q10 pharmacokinetics |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4102142 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267969]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Zhong Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; |
| 推荐引用方式 GB/T 7714 | Wang Yanping,Dai Xiaojian,Li Liang,et al. LC-MS/MS determination of coenzyme Q10 in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2010,30(12):2251-2257. |
| APA | Wang Yanping,Dai Xiaojian,Li Liang,Chen Xiaoyan,&Zhong Dafang.(2010).LC-MS/MS determination of coenzyme Q10 in human plasma.Chinese Journal of Pharmaceutical Analysis,30(12),2251-2257. |
| MLA | Wang Yanping,et al."LC-MS/MS determination of coenzyme Q10 in human plasma".Chinese Journal of Pharmaceutical Analysis 30.12(2010):2251-2257. |
入库方式： OAI收割
来源：上海药物研究所
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