LC-MS/MS determination of voriconazole in low-volume human plasma
文献类型：期刊论文
| 作者 | ZHAO Xiao-hua1; ZHONG Da-fang1 ; SHEN Xiao-hong2; CHEN Xiao-yan1
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| 刊名 | Chinese Journal of New Drugs and Clinical Remedies
 |
| 出版日期 | 2009 |
| 卷号 | 28期号:1页码:43-47 |
| 关键词 | voriconazole chromatography, high pressure liquid spectrometry, mass, electrospray ionization plasma concentration |
| ISSN号 | 1007-7669 |
| 其他题名 | 液相色谱-串联质谱法测定微量人血浆中伏立康唑 |
| 文献子类 | Article |
| 英文摘要 | AIM To develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/ MS) method for quantification of voriconazole (VRC) only using 25 muL human plasma. METHODS Voriconazole and the internal standard, fluconazol, were isolated from 25 muL volume of plasma by liquid-liquid extraction with ether-dichloromethane (3 : 2, V : V), then chromatographed on a Zorbax Extend C_(18) column (150 mm * 4.6 mm I.D., 5 mum, USA) using a mobile phase of acetonitrile-water-formic acid (80 : 20 : 0.15, V : V : V) at a flow rate of 0.50 mL·min~(-1). A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in positive ion mode. Quantification was performed using selected reaction monitoring (SRM) mode of the transition of m/z 350m/z (127 + 281) for VRC and m/z 307m/z (220 + 238) for fluconazol (IS), respectively. RESULTS The complete elution was obtained in less than 4.5 min. The linear concentration ranges of the calibration curves for VRC was 5.00 - 5 000 mug · L~(-1). The lower limit of quantitation of VRC was 5.00 mug·L~(-1). The average extraction recovery was about 90% (RSD ＜ 6.2%). The intra-day and inter-day relative standard deviations were less than 6.6%. CONCLUTION The method is accurate, sensitive, and simple for the pharmacokinetic or bioequivalence study of VRC. The accuracy was within (-1.1 - 1.8)%. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3739587 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267980]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | CHEN Xiao-yan |
| 作者单位 | 1.Shanghai Institute of Materia Mediea, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | ZHAO Xiao-hua,ZHONG Da-fang,SHEN Xiao-hong,et al. LC-MS/MS determination of voriconazole in low-volume human plasma[J]. Chinese Journal of New Drugs and Clinical Remedies,2009,28(1):43-47. |
| APA | ZHAO Xiao-hua,ZHONG Da-fang,SHEN Xiao-hong,&CHEN Xiao-yan.(2009).LC-MS/MS determination of voriconazole in low-volume human plasma.Chinese Journal of New Drugs and Clinical Remedies,28(1),43-47. |
| MLA | ZHAO Xiao-hua,et al."LC-MS/MS determination of voriconazole in low-volume human plasma".Chinese Journal of New Drugs and Clinical Remedies 28.1(2009):43-47. |
入库方式： OAI收割
来源：上海药物研究所
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