LC-MS/MS determination of nisoldipine in human plasma
文献类型：期刊论文
| 作者 | MA Jin-fei1; DAI Xiao-jian2 ; CHEN Xiao-yan2; ZHONG Da-fang1
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2009 |
| 卷号 | 29期号:10页码:1597-1601 |
| 关键词 | nisoldipine liquid chromatography - tandem mass spectrometry plasma drug concentration |
| ISSN号 | 0254-1793 |
| 其他题名 | 液相色谱-串联质谱法测定人血浆中的尼索地平 |
| 文献子类 | Article |
| 英文摘要 | Objective: To develop and validate a liquid chromatography - tandem mass spectrometric ( LC - MS/ MS) method for the determination of nisoldipine in human plasma. Method:The plasma sample was extracted with n -hexane -dichlormethane -isopropanol (20:10:1 ) ,then separated on a Zorbax C_8 ( 150 mm * 4. 6 mm,5 mum) column using acetonitrile - water - formic acid (90: 10:0. 1) as the mobile phase. The triple quadrupole mass spec-trometry was applied via an atmospheric pressure chemical ionization ( APCI) source for detection. Quantitation was performed using multiple reaction monitoring ( MRM) of the transitions of m/z 389. 2315. 2 and m/z 419. 2 343. 2 for nisoldipine and the internal standard nimodipine,respectively. Results;The linear calibration curves were obtained in the concentration range of 0. 020 - 8. 00 ng · mL~(-1). The lower limit of quantification was 0. 020 ng · mL~(-1) . The intra - day and inter - day relative standard deviation over the entire concentration range was less than 7. 6%. The accuracy was in the range of - 3. 4% to 5. 7% in terms of relative error. Each sample was chromato-graphed within 3. 0 min. The method is applied to evaluate the bioequivalence of two nisoldipine tablet formulations in healthy volunteers. Conclusion: This method is sensitive, selective, rapid and suitable for the determination of nisoldipine in human plasma. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3719233 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268027]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | ZHONG Da-fang |
| 作者单位 | 1.Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | MA Jin-fei,DAI Xiao-jian,CHEN Xiao-yan,et al. LC-MS/MS determination of nisoldipine in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2009,29(10):1597-1601. |
| APA | MA Jin-fei,DAI Xiao-jian,CHEN Xiao-yan,&ZHONG Da-fang.(2009).LC-MS/MS determination of nisoldipine in human plasma.Chinese Journal of Pharmaceutical Analysis,29(10),1597-1601. |
| MLA | MA Jin-fei,et al."LC-MS/MS determination of nisoldipine in human plasma".Chinese Journal of Pharmaceutical Analysis 29.10(2009):1597-1601. |
入库方式： OAI收割
来源：上海药物研究所
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